MICHAELIS MENTON KINETICS FOR DETERMINING ENZYMATIC ACTIVITY OF N-ACETYL-BETA-D-GLUCOSAMINIDASE ENZYME IN BLOOD PLASMA OF WILD TYPE INDIVIDUAL

ABSTRACT

Lysosomal storage disorders (LSD) is one of the major groups of genetic disorders with over 40 different disorders that results from the failure of the lysosomal enzymes to function properly. MPS IIIB a common LSD is caused due to deficiency of a-N-Acetylglucosaminidase, which is required for the stepwise degradation of heparan sulfate within the lysosomes. Initially NAGLU enzyme activity increases with increase in substrate concentration and then becomes constant following the Michaelis-Menten kinetics. Further, the NAGLU enzyme activity decreases with increases in incubation time.