The Fluorescence Lifetime Plate Reader (FLPR), designed in collaboration with Fluorescence Innovations, Inc., uses technology similar to that of the TRF instrument in Fargoland, to perform high-throughput time-resolved fluorescence lifetime measurements in a microplate format (96 or 384 wells).
The FLPR is currently equipped with microchip laser excitation at 532 nm and 355 nm, and it records 96 nanosecond time-resolved waveforms within 1 min, the same speed as a conventional intensity-based plate reader, with 10 times better precision.
It will soon be upgraded to include pulsed diode laser excitation at 405 nm and 475 nm and a faster motor, allowing it to read a 384-well plate in 1 min. The FLPR is used by the MN-FLAG (Minnesota Fluorescence Lifetime Assay Group) to develop and apply high-throughput screening assays for drug discovery. The 10-fold increase in precision makes possible HTS assays with unprecedented reliability and resolution.