Targeted Proteomics

  • Cultured dental plaque samples were used as a surrogate for the oral environment.
  • Metaproteomics identified metabolic pathways differentially regulated in response to diet.
  • Selected reaction monitoring (SRM) used to quantify conserved peptides from three enzymes which increase in abundance in sucrose-induced dysbiosis.
  • Shown: NADP-specific glutamate dehydrogenase (EC strongly upregulated in response to sucrose.

Samples not exposed to sucroseSamples grown with sucrose