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Facilities and Techniques

 

Research Facilities

Biophysical Technology Center
  • Electron Paramagnetic Resonance (EPR)
    • Steady-state (continuous wave X-band)
    • Time-resolved (pulsed X-band, DEER)
  • Fluorescence and Luminescence Resonance Energy Transfer (FRET and LRET)
    • Steady-state (Eclipse, Gemini)
    • Time-resolved (Direct Waveform Recording, Single Photon Counting, DWR Flourescence Lifetime Plate Reader)
  • Time-resolved Phosphorescence Anisotropy (TPA)
Nuclear Magnetic Resonance Facility
Peptide Synthesis Facility

Techniques

Computation
Crystallography
Enzyme Kinetics
  • Correlate biochemical and molecular dynamics
  • Transient kinetics using laser flash photolysis
  • Transient Time-resolved FRET
Physiology
Protein Engineering
  • Molecular Biology:
    • Site-directed mutagenesis
    • site-directed labeling
    • fluorescent fusion proteins
  • Cell culture: bacteria, Dictyostelium, insect, yeast, mammalian