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Polymerase Chain Reaction (PCR)

 

For a 50 µl reaction, use:
5 µl 10x PCR buffer
7 µl 25 mM MgCl2
2 µl dNTP
1 µl primer A
1 µl primer B
1 µl Taq polymerase
1 µl template DNA
32 µl ddH2O
 
10x PCR buffer:
5 parts 1 M KCl
1 part 1 M Tris, pH 8.0
4 parts ddH2O