Media Recipes


LB broth, agar

  • 10g Tryptone
  • 5g Yeast Extract
  • 5g NaCl
  • 100µL of 10 M NaOH
  • H2O to 1L
    • For agar add 15g of bacto-agar
  • Autoclave to sterilize

NZY broth, agar, top agarose

  • 5g NaCl
  • 2g MgSO4*7H2O
  • 5g Yeast Extract
  • 10g NZ Amine-A (casein hydrolysate)
  • 300µL of 10 M NaOH
  • H2O to 1L
    • For agar add 15g of bacto-agar
    • For top agarose add 7g of agarose
  • Autoclave to sterilize

NZYM broth, agar, top agarose

  • Prepare NZY broth, agar, or top agarose.
  • After autoclaving, add 50 mL of 20% Maltose per Liter of NZY
    • Autoclaving can oxidize maltose and other sacharides. Therefore, the 20% maltose stock solution must be filter sterilized to prevent contamination.