We are combining top-down and bottom-up approaches to synthetic biology; we use tools of protein engineering and molecular biology, together with novel synthetic cell technologies, to understand and modulate biological processes in complex systems.
We study the genetics of complex traits: how variation in genome sequences influences individual molecular, cellular and organismal features. We also seek to understand the evolutionary forces that shape genomic diversity.
T2D is the most common chronic disease affecting human health, affecting more than 347 million individuals worldwide. T2D is a complex disease characterized by pancreatic β-cell failure to make sufficient amounts of insulin in the setting of obesity and insulin resistance in peripheral tissues. The risk for T2D is determined by many factors. Our genetic makeup can predispose us for T2D, and lifestyle can also reduce or exacerbate health risks. But in recent years, our studies and those of others have shown the importance of babies' time in the womb for influencing health over our lifespan. We and others have shown that poor uterine environments can increase the risk of many diseases in adulthood, including diabetes. Therefore, the long-term goal of the Alejandro Lab is to stop the vicious cycle of diabetes by launching a two-pronged research program for both intervention and prevention of this disease:
Transcriptional regulation in cancer and development
|Transplant immune responses/therapy and use of induced pluripotent stem cells|
Population, evolutionary, and medical genomics; understanding how human genetic variation affects phenotypic diversity and complex disease; computational genomics and metagenomics
We investigate mechanisms that coordinate cell behaviors across great distances during tissue morphogenesis.
Cell adhesion, signal transduction, cytoskeleton, and C. elegans.
Yeast and Human Cell Cycle Control
Clathrin-mediated endocytosis; mammalian intracellular membrane trafficking.
Structure, assembly and dynamics of actin-based cytoskeletal network
|Focuses on the role of the androgen receptor (AR) and alterations in AR signaling in prostate cancer development and firstname.lastname@example.org||612-625-1504|
We focus on testing the mutation theory of aging: if accumulation of DNA mutations in normal somatic cells is a causal mechanism to age-related functional decline. We approach this by developing and applying state-of-the-art single-cell multi-omics technologies and machine learning algorithms.
The research in the Engelhart laboratory is directed towards better understanding nucleic acid folding and function in order to advance two broad themes: 1) the development of novel nucleic acid-based imaging, analytical, and diagnostic technologies and 2) the elucidation of unanticipated roles for nucleic acids in vivo.
Molecular Basis of Muscular Dystrophy; Role of Actin in Cell Polarity
Signal transduction and lymphocyte development
Cytoskeleton and Cell Motility, developmental mechanisms, neuroscience, and regulation of gene expression
Chromatin mechanics and dynamics; Quantitative fluorescence microscopy
Regenerative medicine, cardiogenesis, and stem-cell biology.
We are interested in discovering novel signaling pathways and mechanisms that affect development and aging in the nematode Caenorhabditis elegans using a combination of genetic, drug screening and biochemical approaches.
Fundamental and fascinating developmental processes of meiosis and fertilization using C. elegans
How Rb/E2F1 mediated apoptosis is regulated in normal and cancer cells
The causes and consequences of programmed mutagenesis
Cytoskeleton and cell motility, developmental mechanisms
Molecular mechanism of cell-fate determination in T cells
Kidney development, transcriptional regulation, microRNAs, primary cilia, polycystic kidney disease (PKD)
Development, homeostasis and trafficking of T lymphocytes
|Junge, Harald||Retina, neurovascular interactions, wnt email@example.com||612-624-6017|
Understanding the molecular and genetic mechanisms of vertebrate limb development and apply the study to elucidate the mechanisms of congenital limb in human and limb regeneration
Nuclear reprogramming in somatic cell nuclear cloning and stem cells
Our computational microbiology lab develops methods that bring precision medicine to the microbiome. We apply those methods to find patterns in microbial communities that predict and diagnose human diseases, and we use those patterns to develop novel therapeutics and diagnostics.
Cell cycle regulation, Ubiquitination and proteolysis, Genetic mechanisms of tumorigenesis
Stem Cell Biology: regulatory pathways, diseases and therapies Transcriptional control of mesoderm development
The Lange lab studies how steroid hormone receptor (SR) positive and hormone-influenced cancers escape molecular targeted therapies. Signal transduction is an essential role of SRs. Indeed, all SRs can rapidly activate cytoplasmic protein kinases and act as “growth factor sensors”. In this role, SRs are heavily phosphorylated by mitogenic protein kinases (MAPKs, AKT, CDKs) that are frequently elevated and activated in SR+ (breast and reproductive tract) cancers. Phosphorylation of SRs alters their binding partners and promoter selection and influences cancer cell fate/plasticity by regulating genes that specify proliferative, pro-survival, metabolic, and cancer stem cell programs. Identifying the required kinases and co-activator partners of phospho-SRs will enable the targeting of multiple signaling molecules in addition to SRs, which is predicted to halt cancer metastasis, prevent recurrence, and increase patient survival.
Identification and understanding of genes involved in cancer development
Focus on signaling and transcriptional mechanisms that regulate osteoclast differentiation
Cell and molecular biology of HIV and HTLV
Gene therapy for genetic disease and cancer using viral and non-viral vectors
Craniofacial muscles in health and disease
Integrative systems biology of cardiovascular function; Cardiac gene therapy; Transgenic models of heart disease; Molecular mechanisms of sarcomere function; Human iPS cell cardiac myocytes
My lab works on pediatric cancer genetics, immunotherapy, and gene therapy using cutting edge technologies, including DNA transposons, TALENs, and CRISPR/Cas9.
Computational biology and functional genomics - Machine learning for integrating diverse genomic data to make inferences about biological networks
Molecular and genetic analysis of Drosophila development
Developmental control of growth and cell proliferation in Drosophila
Cell-cell interactions in growth, differentiation, and development
Mechanisms controlling lineage decision and reprogramming, and application to regenerative medicine
Regulation of dynein-based motility
Our group invents and applies protein engineering technologies to study fundamental functional principles of natural and artificial living systems at a cellular level.
Lymphocyte and tumor cell adhesion, migration and signal transduction
Animal development; control of gene expression; chromatin mechanisms
Protein acrobatics - Study of protein function via protein engineering; Focus on cell signaling and motor proteins
My laboratory is interested in understanding the lifecycle of retroviruses and use this information 1) to identify new drug targets for HIV, 2) to develop better vectors for gene therapy and 3) to use these vectors for gene discovery.
My research focuses mainly on the roles of microRNAs in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), obesity, insulin resistance and liver cancer, with the goal to develop novel therapeutic approaches for these disorders.
Understanding the genetics of cancer in order to develop individualized, targeted therapies
Molecular genetic analysis of unconventional myosin function
Stem cell gene therapy
Role of cardiac progenitor cells in vivo during cardiac development
Cancer transgenic mouse models and pharmacogenomics
Plant genome engineering through homologous recombination; Retrotransposable elements and genome organization
Evaluate retroviral-mediated gene transfer in hematopoietic cells and reversal of the disease process in vitro thus providing the impetus to initiate clinical trials of gene therapy
Cancer gene-therapy and virotherapy
Molecular genetics of sexual differentiation, germ cell development, and testis cancer
Structure and function of membrane-bound transport proteins
C. albicans genetics and pathogenesis
Transposons, human gene therapy, vertebrate gene expression, mouse, zebrafish
Cytogenetic techniques to elucidate constitutional and acquired chromosome abnormalities
The role of DNA repair and recombination in maintaining genome stability
Axon guidance and growth cone motility
Chromatin, Genome Instability, DNA Repair, Repetitive DNA Sequences, Bioinformatics
Stem cells for therapeutic retinal cell replacement and progenitor cell maturation in retinal development
Mammalian brain development, cell type specification and establishment of neuronal connectivity
Mechanisms by which cells grow and divide
Function of O-GlcNAc protein modification in growth and development, molecular genetics of plant DNA viruses
Developmental timing in C. elegans: from microRNAs to nutritional cues
Desiccation stress, viable but non-culturable cells, biofilms, mRNA stability, plant-pathogen interactions
Uses the laboratory mouse as a model to understand a causative link between chromosome instability and cancer
Discovery and validation of biomarkers for ovarian cancer
Non-viral gene therapy using nanocapsules and the Sleeping Beauty (SB) transposon; antiapoptosis of neurodegenerative disorders using ursodeoxycholic acid as a therapeutic drug; genomic methylation changes associated with transposition of SB; and induced by microRNAs