Fluorescence microscopy is an indispensable technique in the Biological Sciences that is used for studying proteins and cells in their physiological state. While fluorescence microscopy is commonly employed for observing molecules or disease progression, its real strength lies in the ability to reveal quantitative information. The main goal of this course is to introduce the fundamental principles of fluorescence, fluorescence microscopy, and fluorescence quantification to extract maximal information from fluorescence experiments. The course will use FIJI (ImageJ) tutorials along with lectures to provide hands-on expertise in image analysis and interpretation. Students will gain both a broad understanding of fluorescence microscopy techniques. Graduate students will gain confidence in quantitative analysis of their own image data. Undergraduate students who proceed to graduate school, medical school, or employment in the biotech industry will find that quantitative microscopy skills will provide them an essential competitive edge.