Enroll in May Term Course GCD 5111: Quantitative Fluorescence Microscopy

Earn 3 credits in a 3 week intensive online lab course. Learn all you ever wanted to know about microscopy and data analysis but were too afraid to ask. Registration for summer courses opens February 25.

Fluorescence microscopy is an indispensable technique in the Biological Sciences that is used for studying proteins and cells in their physiological state. While fluorescence microscopy is commonly employed for observing molecules or disease progression, its real strength lies in the ability to reveal quantitative information. The main goal of this course is to introduce the fundamental principles of fluorescence, fluorescence microscopy, and fluorescence quantification to extract maximal information from fluorescence experiments. The course will use FIJI (ImageJ) tutorials along with lectures to provide hands-on expertise in image analysis and interpretation. Students will gain both a broad understanding of fluorescence microscopy techniques. Graduate students will gain confidence in quantitative analysis of their own image data. Undergraduate students who proceed to graduate school, medical school, or employment in the biotech industry will find that quantitative microscopy skills will provide them an essential competitive edge.

Course Details

  • Instructor: Sivaraj Sivaramakrishnan
  • Email: sivaraj@umn.edu
  • Department: Genetics, Cell Biology & Development
  • Course Prerequisites: For BIOL 5111 - BIOL 4004 or GCD 3033
  • Credits: 3
  • Meeting Time/Place:
    • 9 am – 1 pm CT (Tu/We/Th/Fr)
    • Online (Canvas)
    • Video lectures and assignments will be posted to the Canvas site by 3 pm of the previous day
    • Students have until 11 am of the day of the module to review video lectures and assignments
    • Group discussion on Canvas: 11 – 12.15 pm
    • Flipgrid video and response: 12.15 – 12.30 pm
    • Zoom discussion: 12.30 – 1 pm


February, 2021