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New Course: Quantitative Fluorescence Microscopy

An essential primer in quantitative fluorescence microscopy for graduate students and senior undergraduates.

Immunofluorescence of keratin intermediate filaments in alveolar cells

GCD 8920/BIOL 5950 • Spring 2017 • 3 credits

Fluorescence microscopy is commonly employed for observing molecules or disease progression. However, its real strength lies in revealing quantitative biological information. The main goal of this course is to introduce the fundamental principles of fluorescence, fluorescence microscopy, and fluorescence quantification to extract maximal information from experimental data. The course will use ImageJ tutorials along with lectures to provide hands-on expertise in image analysis and interpretation. 

Students will gain both a broad understanding of fluorescence microscopy techniques. Graduate students will gain confidence in quantitative analysis of their own image data that is increasingly essential for high-profile publications. Undergraduate students who plan to attend graduate school, medical school, or pursue employment in the biotech industry will find that quantitative microscopy skills will provide them an essential competitive edge.


  • Course meets: Wednesday and Friday, 10 a.m. - 12 p.m.
  • Grading: The course is being offered as satisfactory/no credit (S/N). Class participation and homework assignments based on class participation will account for most of the grade. BIOL 5950 will have a final exam, whereas GCD 8920 will have a mid term exam and a final class presentation.
  • Course Instructor: Sivaraj (Shiv) Sivaramakrishnan (
  • Course website >>


November, 2016