Fluorescence microscopy is commonly employed for observing molecules or disease progression. However, its real strength lies in revealing quantitative biological information. The main goal of this course is to introduce the fundamental principles of fluorescence, fluorescence microscopy, and fluorescence quantification to extract maximal information from experimental data. The course will use ImageJ tutorials along with lectures to provide hands-on expertise in image analysis and interpretation.
Students will gain both a broad understanding of fluorescence microscopy techniques. Graduate students will gain confidence in quantitative analysis of their own image data that is increasingly essential for high-profile publications. Undergraduate students who plan to attend graduate school, medical school, or pursue employment in the biotech industry will find that quantitative microscopy skills will provide them an essential competitive edge.