The Blazar and Tolar Labs have openings for 2 undergrads to start immediately with a potential for a paid position. Students should be interested in a long-term research opportunity and should have completed Foundations of Biology.
There are two main projects, one in the Blazar lab and one in the Tolar lab (these labs share a lab space). Both projects will involve working with human induced pluripotent stem (iPS) cells and genome editing technologies (TALEN/CRISPR/Cas9).
Project 1: T-cell iPS project (Blazar lab)
This project is working to determine the effect of specific genetic modifications on in vitro T-cell development from human iPSC. We are using multiple genome editing strategies, including both TALENs, CRISPR/Cas9 and transposon based systems to accomplish this. The student will have the opportunity to learn advanced molecular cloning techniques, iPS culture and differentiation strategies, as well as strategies to induce and verify specific genetic modifications in human iPSC.
Project 2: Epidermolysis bullosa (EB)
This project aims to develop novel gene corrections strategies (again using TALENs and CRISPR/Cas9) that will be applied to patient derived iPSC. Corrected cell lines are being used to generate a number of therapeutic cell types in vitro. The student will be doing many of the things I mentioned in project 1.
Interested students should email Dr. Beau Webber with a CV, a statement of interest and what you are looking for in a research experience.