The Fluorescence Lifetime Plate Reader (FLPR), designed in collaboration with Fluorescence Innovations, Inc., uses technology similar to that of the TRF instrument in Fargoland, to perform high-throughput time-resolved fluorescence lifetime measurements in a microplate format (96 or 384 wells).
The FLPR is currently equipped with microchip laser excitation at 532 nm and 355 nm, and it records 384 nanosecond time-resolved waveforms within 10 min, the same speed as a conventional intensity-based plate reader, with 30 times better precision.
Selected publications of instrument use:
- Muretta, JM, A Kyrychenko, AS Ladokhin, DJ Kast, GD Gillispie, and DD Thomas. 2010. High -performance time-resolved fluorescence by direct waveform recording. Rev Sci Instrum 81:103101 (1-8).
- Rebbeck, RT, MM Essawy, FR Nitu, BD Grant, GD Gillispie, DD Thomas, DM Bers, and RL Cornea. 2017. High-throughput screens to discover small-molecule modulators of ryanodine receptor calcium release channels. SLAS Discov 22(2):176-186.
The BTC should be acknowledged in any publications arising from the data analyzed in the BTC with the following text:
Experiments reported in this paper were performed at the Biophysical Technology Center, University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics.
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