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Discovery Metabolomics

Elevated Endogenous Peptides in Lung Lavage Fluid Associated with Bronchiolitis Obliterans Syndrome (BOS).

Stone et al., PloS ONE 9(1): e84471, doi:10.1371/jornal.pone.oo84471

  • Mass spectrometry was used to identify low-mass compounds in bronchoalveolar lavage fluid that are associated with BOS.
  • Greater abundance of features with BOS while high inter-sample correlation among controls suggests relatively uniform components in normal BALF.
  • The number of observed features increases closer to diagnosis of BOS.
  • Metabolite/Small Molecule analysis of bronchoalveolar lavage fluid from patients with BOS showed elevated endogenous peptides – consistent with elevated neutrophil elastase activity.
  • Evidence of increased protease activity in BOS is consistent with our published proteomic data demonstrating an increase of matrix metalloproteinase-9 and proteinase-3 in BOS bronchoalveolar lavage fluid.