Elevated Endogenous Peptides in Lung Lavage Fluid Associated with Bronchiolitis Obliterans Syndrome (BOS).
- Mass spectrometry was used to identify low-mass compounds in bronchoalveolar lavage fluid that are associated with BOS.
- Greater abundance of features with BOS while high inter-sample correlation among controls suggests relatively uniform components in normal BALF.
- The number of observed features increases closer to diagnosis of BOS.
- Metabolite/Small Molecule analysis of bronchoalveolar lavage fluid from patients with BOS showed elevated endogenous peptides – consistent with elevated neutrophil elastase activity.
- Evidence of increased protease activity in BOS is consistent with our published proteomic data demonstrating an increase of matrix metalloproteinase-9 and proteinase-3 in BOS bronchoalveolar lavage fluid.