Proteomics Technologies
Discovery-based
- Protein Identification by data dependent peptide tandem MS
- 1D capillary LC-MS/MS
- Thermo Orbitrap Fusion ($95/hr*; appx. 2hr/sample)
- Thermo Orbitrap Velos ($70/hr*; appx. 1.7hr/sample)
- Thermo LTQ linear ion trap ($50/hr*; appx. 1.25hr/sample)
- 1D capillary LC-MS/MS
- Phosphopeptide enrichment; sequential TiO2 > FeNTA ($179.40 per enrichment)
- Quantification and Identification by data-dependent peptide tandem MS
- TMT isobaric labeling technology (TMT 10plex™, TMT 11plex™, soon to offer TMT Pro 16plex™ )
Targeted
- Peptide SRM (selected reaction monitoring) on SCIEX QTRAP 5500, SCIEX QTRAP 6500+, Agilent 6495C
- Peptide PRM (parallel reaction monitoring) on Thermo Orbitrap Fusion and Thermo Orbitrap Eclipse
DIA (Data Independent Acquisition of all Theoretical mass spectra)
- Peptide-level data-independent acquisition using Thermo's Orbitrap Fusion and Eclispe
MALDI-TOF MS (Bruker Autoflex)
- Trained user rate ($82.25/hr*)
- Staff analysis ($48.50/sample*)
Metabolomics Technologies
Discovery-based LC-MS or LC-MS/MS
- Thermo Q Exactive Orbitrap ($120/hr*; appx. 20-30 min/sample)
Discovery-based or Targeted GC-MS
- Agilent 7200 accurate mass GC/QTOF-MS ($62.90/hr*)
Targeted LC-MS
- SRM (selected reaction monitoring) on SCIEX QTRAP 5500, SCIEX QTRAP 6500+, Agilent 6495C
- PRM or SIM on Thermo Q Exactive Orbitrap ($120/hr*)
* University of Minnesota Internal Customer rates
The rates shown below are for samples submitted to the CMSP on or after July 1, 2019.
UMNInternal RATES | ExternalRATES | |
|---|---|---|
INSTRUMENTS
LC-MS | ||
Orbitrap Fusion, Thermo (hourly, full service) | $95.00 | $190.00 |
Orbitrap Velos, Thermo (hourly, full service) | $70.00 | $140.00 |
LTQ, Thermo (hourly, full service) | $50.00 | $100.00 |
Orbitrap Q-Exactive, Thermo (hourly, full service) | $120.00 | $240.00 |
LCQ, Thermo (hourly) | $49.50 | $99.00 |
QTRAP 5500, SCIEX (hourly) | $110.00 | $220.00 |
| QTRAP 6500*, SCIEX (hourly, full service) | $165.00 | $330.00 |
| Triple Quadrupole 6495C, Agilent (hourly, fullservice) | $150.00 | $300.00 |
| Orbitrap Eclipse, Thermo (Hourly, full service) | $165.00 | $330.00 |
GC-MS | ||
7200 Quadrupole Time-of-Flight, Agilent (hourly, full service) | $62.90 | $125.80 |
MALDI-TOF | ||
BRUKER Autoflex (per sample, full service) | $48.50 | $97.00 |
BRUKER Autoflex (per hour, trained user) | $80.25 | $160.50 |
SAMPLE PREPARATION | ||
Method Development (hourly) | $97.56 | $195.12 |
Sample Preparation (hourly) | $80.47 | $160.94 |
Protein Quantification Assay (per set of samples) | $80.47 | $160.94 |
Barocycler use and consumables (per sample) | $15.20 | $30.40 |
Off-line LC (C18 high pH) (per run) | $275.00 | $550.00 |
Phosphorylation Enrichment, Fe-NTA and TiO2 column (Pierce) (per sample) | $179.40 | $358.80 |
PROTEOLYTIC DIGESTIONS AND SDS-PAGE | ||
SDS-PAGE gel analysis | $92.50 | $185.00 |
Trypsin vial | $14.96 | $29.92 |
Digestion in-gel (full service, 10 samples) | $165.00 | $330.00 |
Digestion in-gel (cost per band after 10) | $13.00 | $26.00 |
Trypsin digest in-solution (full service, per sample) | $20.00 | $40.00 |
Asp-N enzyme (add-on to trypsin digest) | $148.58 | $297.16 |
GluC enzyme (add-on to trypsin digest) | $50.28 | $100.56 |
Chymotrypsin enzyme (add-on to trypsin digest) | $42.94 | $85.88 |
Trypsin/Lys-C enzyme (add-on to trypsin digest) | $25.84 | $51.68 |
Lys-C enzyme (add-on to trypsin digest) | $119.43 | $238.86 |
Pepsin enzyme (add-on to trypsin digest) | $1.00 | $2.00 |
ISOTOPE LABELING | ||
| TMT 10plex™ (Thermo) reagent only - Phospho enrichment + Global analysis | $1086.80 | $2173.60 |
TMT 10plex™ (Thermo) reagent only - Global analysis (no enrichment) | $562.50 | $1125.00 |
iTRAQ® 8plex (SCIEX) reagent, labeling, sample prep | $767.80 | $1535.60 |
iTRAQ® 4plex (SCIEX) reagent, labeling, sample prep | $625.90 | $1251.80 |
SAMPLE CLEAN-UP | ||
ZipTip (consumable only) | $3.00 | $6.00 |
ZipTip (Millipore) (full service) | $25.00 | $50.00 |
Stage Tip (C18 or MCX) (full service) | $13.16 | $26.32 |
SPE Stage Tip (C18 or MCX) (consumable only) | $0.50 | $1.00 |
SPE MCX Cartridge (full service) | $25.00 | $50.00 |
SPE MW 3K cutoff (column only) | $3.30 | $6.60 |
SPE C18 (full service) | $25.00 | $50.00 |
SPE C18 1 cc (column only) | $1.56 | $3.12 |
SPE C18 3 cc (column only) | $2.48 | $4.96 |
SPE MCX 1 cc (column only) | $1.98 | $3.96 |
SPE MCX 3 cc (column only) | $2.85 | $5.70 |
DATA ANALYSIS | ||
Data Analysis (hourly) | $116.14 | $232.28 |