Proteomics Technologies
Discovery-based
- Protein Identification by data dependent peptide tandem MS
- 1D capillary LC-MS/MS
- Thermo Orbitrap Fusion ($65/hr*; appx. 2hr/sample) (Cost estimate: 6 samples)
- Thermo Orbitrap Velos ($55/hr*; appx. 1.7hr/sample)
- Thermo LTQ linear ion trap ($40/hr*; appx. 1.25hr/sample)
- 1D capillary LC-MS/MS
- Phosphopeptide enrichment; sequential TiO2 > FeNTA (Cost estimate, 9 samples)
- Quantification and Identification by data-dependent peptide tandem MS
- TMT isobaric labeling technology (Cost estimate TMT 10plex™)
- iTRAQ® isobaric labeling technology (Cost estimate iTRAQ 4plex)
Targeted
- Peptide SRM (selected reaction monitoring) on QTRAP 5500 ($81.40/hr*)
- Peptide PRM (parallel reaction monitoring) on Thermo Orbitrap Fusion ($65/hr*)
SWATH (Sequential Window Acquisition of all Theoretical mass spectra)
- Peptide-level data-independent acquisition on SCIEX 5600 Triple TOF ($65/hr*)
MALDI-TOF MS (Bruker Autoflex)
- Trained user rate ($77/hr*)
- Staff analysis ($38.50/sample*)
Metabolomics Technologies
Discovery-based LC-MS or LC-MS/MS
- Thermo Q Exactive Orbitrap ($94.60/hr*; appx. 20-30 min/sample)
Discovery-based or Targeted GC-MS
- Agilent 7200 accurate mass GC/QTOF-MS ($45/hr*)
Targeted LC-MS
- SRM (selected reaction monitoring) on SCIEX QTRAP 5500 ($81.40/hr*)
- PRM or SIM on Thermo Q Exactive Orbitrap ($94.60/hr*)
* University of Minnesota Internal Customer rates
The rates shown below are for samples submitted to the CMSP on or after July 1, 2019.
UMNInternal RATES | ExternalRATES | |
|---|---|---|
INSTRUMENTS
LC-MS | ||
Orbitrap Fusion, Thermo (hourly, full service) | $65.00 | $130.00 |
Orbitrap Velos, Thermo (hourly, full service) | $55.00 | $110.00 |
LTQ, Thermo (hourly, full service) | $40.00 | $80.00 |
Orbitrap Q-Exactive, Thermo (hourly, full service) | $94.60 | $189.20 |
LCQ, Thermo (hourly) | $49.50 | $99.00 |
QTRAP 5500, SCIEX (hourly) | $81.40 | $162.80 |
Triple-TOF 5600, SCIEX (hourly, full service) | $65.00 | $130.00 |
GC-MS | ||
7200 Quadrupole Time-of-Flight, Agilent (hourly, full service) | $45.00 | $90.00 |
MALDI-TOF | ||
BRUKER Autoflex (per sample, full service) | $38.50 | $77.00 |
BRUKER Autoflex (per hour, trained user) | $77.00 | $154.00 |
SAMPLE PREPARATION | ||
Method Development (hourly) | $68.75 | $137.50 |
Sample Preparation (hourly) | $68.75 | $137.50 |
Protein Quantification Assay (per set of samples) | $68.75 | $137.50 |
Barocycler use and consumables (per sample) | $13.20 | $26.40 |
Off-line LC (C18 high pH) (per run) | $136.40 | $272.80 |
Fe-NTA column (Pierce) | $11.37 | $22.74 |
TiO2 column (Pierce) | $18.57 | $37.14 |
PROTEOLYTIC DIGESTIONS AND SDS-PAGE | ||
SDS-PAGE gel analysis | $82.50 | $165.00 |
Trypsin vial | $14.96 | $29.92 |
Digestion in-gel (full service, 10 samples) | $132.00 | $264.00 |
Digestion in-gel (cost per band after 10) | $11.00 | $22.00 |
Trypsin digest in-solution (full service, per sample) | $82.50 | $165.00 |
Asp-N enzyme (add-on to trypsin digest) | $148.58 | $297.16 |
GluC enzyme (add-on to trypsin digest) | $50.28 | $100.56 |
Chymotrypsin enzyme (add-on to trypsin digest) | $42.94 | $85.88 |
Trypsin/Lys-C enzyme (add-on to trypsin digest) | $25.84 | $51.68 |
Lys-C enzyme (add-on to trypsin digest) | $119.43 | $238.86 |
Pepsin enzyme (add-on to trypsin digest) | $1.00 | $2.00 |
ISOTOPE LABELING | ||
| TMT 10plex™ (Thermo) reagent only - Phospho enrichment + Global analysis | $996.00 | $1992.00 |
TMT 10plex™ (Thermo) reagent only - Global analysis (no enrichment) | $520.00 | $1040.00 |
iTRAQ® 8plex (SCIEX) reagent, labeling, sample prep | $767.80 | $1535.60 |
iTRAQ® 4plex (SCIEX) reagent, labeling, sample prep | $625.90 | $1251.80 |
SAMPLE CLEAN-UP | ||
ZipTip (consumable only) | $3.00 | $6.00 |
ZipTip (Millipore) (full service) | $16.50 | $33.00 |
Stage Tip (C18 or MCX) (full service) | $10.16 | $20.32 |
SPE Stage Tip (C18 or MCX) (consumable only) | $0.50 | $1.00 |
SPE MCX Cartridge (full service) | $14.40 | $28.80 |
SPE MW 3K cutoff (column only) | $3.30 | $6.60 |
SPE C18 (full service) | $17.06 | $34.12 |
SPE C18 1 cc (column only) | $1.56 | $3.12 |
SPE C18 3 cc (column only) | $2.48 | $4.96 |
SPE MCX 1 cc (column only) | $1.98 | $3.96 |
SPE MCX 3 cc (column only) | $2.85 | $5.70 |
DATA ANALYSIS | ||
Data Analysis (hourly) | $68.75 | $137.50 |