CMSP Technologies & Service Fees

 

Proteomics Technologies

Discovery-based

  • Protein Identification by data dependent peptide tandem MS
    • 1D capillary LC-MS/MS
      • Thermo Orbitrap Fusion ($65/hr*; appx. 2hr/sample) (Cost estimate: 6 samples)
      • Thermo Orbitrap Velos ($55/hr*; appx. 1.7hr/sample)
      • Thermo LTQ linear ion trap ($40/hr*; appx. 1.25hr/sample)
  • Phosphopeptide enrichment; sequential TiO2 > FeNTA (Cost estimate, 9 samples)
  • Quantification and Identification by data-dependent peptide tandem MS

Targeted

  • Peptide SRM (selected reaction monitoring) on QTRAP 5500 ($81.40/hr*)
  • Peptide PRM (parallel reaction monitoring) on Thermo Orbitrap Fusion ($65/hr*)

SWATH (Sequential Window Acquisition of all Theoretical mass spectra)

  • Peptide-level data-independent acquisition on SCIEX 5600 Triple TOF ($65/hr*)

MALDI-TOF MS (Bruker Autoflex)

  • Trained user rate ($77/hr*)
  • Staff analysis ($38.50/sample*)

Metabolomics Technologies

Discovery-based LC-MS or LC-MS/MS 

  • Thermo Q Exactive Orbitrap ($94.60/hr*; appx. 20-30 min/sample)

Discovery-based or Targeted GC-MS

  • Agilent 7200 accurate mass GC/QTOF-MS ($45/hr*)

Targeted LC-MS

  • SRM (selected reaction monitoring) on SCIEX QTRAP 5500 ($81.40/hr*)
  • PRM or SIM on Thermo Q Exactive Orbitrap ($94.60/hr*)

* University of Minnesota Internal Customer rates

 

The rates shown below are for samples submitted to the CMSP on or after July 1, 2019.

 

UMN

Internal RATES

External

RATES

INSTRUMENTS

 

LC-MS
  
Orbitrap Fusion, Thermo (hourly, full service)
$65.00
$130.00
Orbitrap Velos, Thermo (hourly, full service)
$55.00
$110.00
LTQ, Thermo (hourly, full service) 
$40.00
$80.00
Orbitrap Q-Exactive, Thermo (hourly, full service)
$94.60
$189.20
LCQ, Thermo (hourly)
$49.50
$99.00
QTRAP 5500, SCIEX (hourly)
$81.40
$162.80
Triple-TOF 5600, SCIEX (hourly, full service)
$65.00
$130.00
GC-MS
  
7200 Quadrupole Time-of-Flight, Agilent (hourly, full service)
$45.00
$90.00
MALDI-TOF
 
 
BRUKER Autoflex (per sample, full service)
$38.50
$77.00
BRUKER Autoflex (per hour, trained user)
$77.00 
$154.00
SAMPLE PREPARATION
  
Method Development (hourly)
$68.75
$137.50
Sample Preparation (hourly)
$68.75
$137.50
Protein Quantification Assay (per set of samples)
$68.75
$137.50
Barocycler use and consumables (per sample)
 $13.20
$26.40
Off-line LC (C18 high pH) (per run)
$136.40
$272.80
Fe-NTA column (Pierce)
$11.37
$22.74
TiO2 column (Pierce)
$18.57
$37.14
PROTEOLYTIC DIGESTIONS AND SDS-PAGE
  
SDS-PAGE gel analysis
$82.50
$165.00
Trypsin vial
$14.96
$29.92
Digestion in-gel (full service, 10 samples)
$132.00
$264.00
Digestion in-gel (cost per band after 10)
$11.00
$22.00
Trypsin digest in-solution (full service, per sample)                                         
$82.50
$165.00
Asp-N enzyme (add-on to trypsin digest)
$148.58
  $297.16
GluC enzyme (add-on to trypsin digest)
$50.28
$100.56
Chymotrypsin enzyme (add-on to trypsin digest)                                         
$42.94
$85.88
Trypsin/Lys-C enzyme (add-on to trypsin digest)
$25.84
$51.68
Lys-C enzyme (add-on to trypsin digest)
$119.43
$238.86
Pepsin enzyme (add-on to trypsin digest)
$1.00
$2.00
ISOTOPE LABELING
  
TMT 10plex™ (Thermo) reagent only - Phospho enrichment + Global analysis$996.00$1992.00
TMT 10plex™ (Thermo) reagent only - Global analysis (no enrichment)
$520.00
$1040.00
iTRAQ® 8plex (SCIEX) reagent, labeling, sample prep
$767.80
$1535.60
iTRAQ® 4plex (SCIEX) reagent, labeling, sample prep
$625.90
$1251.80
SAMPLE CLEAN-UP
  
ZipTip (consumable only)
$3.00
$6.00
ZipTip (Millipore) (full service)
$16.50
$33.00
Stage Tip (C18 or MCX) (full service)
$10.16
$20.32
SPE Stage Tip (C18 or MCX) (consumable only)
$0.50
$1.00
SPE MCX Cartridge (full service)
$14.40
$28.80
SPE MW 3K cutoff (column only)
$3.30
$6.60
SPE C18 (full service)
$17.06
$34.12
SPE C18 1 cc (column only)
$1.56
$3.12
SPE C18 3 cc (column only)
$2.48
$4.96
SPE MCX 1 cc (column only)
$1.98
$3.96
SPE MCX 3 cc (column only)
$2.85
$5.70
DATA ANALYSIS
 
 
Data Analysis (hourly)
$68.75
$137.50