Proteomics Technologies
Discovery-based
- Protein Identification by data dependent peptide tandem MS
- 1D capillary LC-MS/MS
- Thermo Orbitrap Eclipse ($130 /hr*; appx. 2hr/sample)
- Thermo Orbitrap Fusion ($95/hr*; appx. 2hr/sample)
- Thermo Orbitrap Velos ($70/hr*; appx. 1.7hr/sample)
- Thermo LTQ linear ion trap ($50/hr*; appx. 1.25hr/sample)
- 1D capillary LC-MS/MS
- Phosphopeptide enrichment; sequential TiO2 > FeNTA ($179.40 per enrichment)
- Quantification and Identification by data-dependent peptide tandem MS
- TMT isobaric labeling technology (TMT 10plex™, TMT 11plex™, TMT Pro 16plex™ )
Targeted
- Peptide SRM (selected reaction monitoring) on SCIEX QTRAP 5500, SCIEX QTRAP 6500+, Agilent 6495C
- Peptide PRM (parallel reaction monitoring) on Thermo Orbitrap Fusion and Thermo Orbitrap Eclipse
DIA (Data Independent Acquisition of all Theoretical mass spectra)
- Peptide-level data-independent acquisition using Thermo's Orbitrap Fusion and Eclispe
MALDI-TOF MS (Bruker Autoflex)
- Trained user rate ($82.25/hr*)
- Staff analysis ($48.50/sample*)
Metabolomics Technologies
Discovery-based LC-MS or LC-MS/MS
- Thermo Q Exactive Orbitrap ($120/hr*; appx. 20-30 min/sample)
Discovery-based or Targeted GC-MS
- Agilent 7200 accurate mass GC/QTOF-MS ($62.90/hr*)
Targeted LC-MS
- SRM (selected reaction monitoring) on SCIEX QTRAP 5500, SCIEX QTRAP 6500+, Agilent 6495C
- PRM or SIM on Thermo Q Exactive Orbitrap ($120/hr*)
* University of Minnesota Internal Customer rates
CMSP FY22 Rates can be found HERE (starting Aug 15th, 2021)
The rates shown below are for samples submitted to the CMSP on or after July 1, 2020.
| Instruments | UMN Internal RATES | External RATES |
|---|---|---|
LC-MS |
||
| Orbitrap Fusion, Thermo (hourly, full service) | $95.00 | $190.00 |
| Orbitrap Fusion - Reduced Rate , Thermo (Hourly, >30 hrs of run time in one experiment) | $67.96 | $135.92 |
| Orbitrap Velos, Thermo (hourly, full service) | $70.00 | $140.00 |
| LTQ, Thermo (hourly, full service) | $50.00 | $100.00 |
| Orbitrap Q-Exactive, Thermo (hourly, full service) | $120.00 | $240.00 |
| LCQ, Thermo (hourly) | $49.50 | $99.00 |
| QTRAP 5500, SCIEX (hourly) | $110.00 | $220.00 |
| QTRAP 6500*, SCIEX (hourly, full service) | $130.00 | $260.00 |
| Triple Quadrupole 6495C, Agilent (hourly, fullservice) | $130.00 | $260.00 |
| Orbitrap Eclipse, Thermo (Hourly, full service) | $130.00 | $260.00 |
| Orbitrap Eclipse - Reduced Rate , Thermo (Hourly, >30 hrs of run time in one experiment) | $96.77 | $193.54 |
GC-MS |
||
| 7200 Quadrupole Time-of-Flight, Agilent (hourly, full service) | $62.90 | $125.80 |
MALDI-TOF |
||
| BRUKER Autoflex (per sample, full service) | $48.50 | $97.00 |
| BRUKER Autoflex (per hour, trained user) | $80.25 | $160.50 |
SAMPLE PREPARATION |
||
| Method Development (hourly) | $97.56 | $195.12 |
| Sample Preparation (hourly) | $80.47 | $160.94 |
| Protein Quantification Assay (per set of samples) | $80.47 | $160.94 |
| Barocycler use and consumables (per sample) | $15.20 | $30.40 |
| Off-line LC (C18 high pH) (per run) | $275.00 | $550.00 |
| Phosphorylation Enrichment, Fe-NTA and TiO2 column (Pierce) (per sample) | $179.40 | $358.80 |
PROTEOLYTIC DIGESTIONS AND SDS-PAGE |
||
| SDS-PAGE gel analysis | $92.50 | $185.00 |
| Trypsin vial | $14.96 | $29.92 |
| Digestion in-gel (full service, 10 samples) | $165.00 | $330.00 |
| Digestion in-gel (cost per band after 10) | $13.00 | $26.00 |
| Trypsin digest in-solution (full service, per sample) | $20.00 | $40.00 |
| Asp-N enzyme (add-on to trypsin digest) | $148.58 | $297.16 |
| GluC enzyme (add-on to trypsin digest) | $50.28 | $100.56 |
| Chymotrypsin enzyme (add-on to trypsin digest) | $42.94 | $85.88 |
| Trypsin/Lys-C enzyme (add-on to trypsin digest) | $25.84 | $51.68 |
| Lys-C enzyme (add-on to trypsin digest) | $119.43 | $238.86 |
| Pepsin enzyme (add-on to trypsin digest) | $1.00 | $2.00 |
ISOTOPE LABELING |
||
| TMT 10plex™ (Thermo) reagent only - Phospho enrichment + Global analysis | $1086.80 | $2173.60 |
| TMT 10plex™ (Thermo) reagent only - Global analysis (no enrichment) | $562.50 | $1125.00 |
| TMT-PRO™ (Thermo) reagent only - Global analysis (no enrichment) | $767.80 | $1535.60 |
SAMPLE CLEAN-UP |
$1220.00 | $2440.00 |
| ZipTip (consumable only) | $3.00 | $6.00 |
| ZipTip (Millipore) (full service) | $25.00 | $50.00 |
| Stage Tip (C18 or MCX) (full service) | $13.16 | $26.32 |
| SPE Stage Tip (C18 or MCX) (consumable only) | $0.50 | $1.00 |
| SPE MCX Cartridge (full service) | $25.00 | $50.00 |
| SPE MW 3K cutoff (column only) | $3.30 | $6.60 |
| SPE C18 (full service) | $25.00 | $50.00 |
| SPE C18 1 cc (column only) | $1.56 | $3.12 |
| SPE C18 3 cc (column only) | $2.48 | $4.96 |
| SPE MCX 1 cc (column only) | $1.98 | $3.96 |
| SPE MCX 3 cc (column only) | $2.85 | $5.70 |
DATA ANALYSIS |
||
| Data Analysis (hourly) | $116.14 | $232.28 |