CMSP Technologies & Service Fees

 

Proteomics Technologies

Discovery-based

  • Protein Identification by data dependent peptide tandem MS
    • 1D capillary LC-MS/MS
      • Thermo Orbitrap Fusion ($95/hr*; appx. 2hr/sample) 
      • Thermo Orbitrap Velos ($70/hr*; appx. 1.7hr/sample)
      • Thermo LTQ linear ion trap ($50/hr*; appx. 1.25hr/sample)
  • Phosphopeptide enrichment; sequential TiO2 > FeNTA ($179.40 per enrichment)
  • Quantification and Identification by data-dependent peptide tandem MS
    • TMT isobaric labeling technology (TMT 10plex™, TMT 11plex™, soon to offer TMT Pro 16plex™ )

Targeted

  • Peptide SRM (selected reaction monitoring) on SCIEX QTRAP 5500, SCIEX QTRAP 6500+, Agilent 6495C
  • Peptide PRM (parallel reaction monitoring) on Thermo Orbitrap Fusion and Thermo Orbitrap Eclipse

DIA (Data Independent Acquisition of all Theoretical mass spectra)

  • Peptide-level data-independent acquisition using Thermo's Orbitrap Fusion and Eclispe

MALDI-TOF MS (Bruker Autoflex)

  • Trained user rate ($82.25/hr*)
  • Staff analysis ($48.50/sample*)

Metabolomics Technologies

Discovery-based LC-MS or LC-MS/MS 

  • Thermo Q Exactive Orbitrap ($120/hr*; appx. 20-30 min/sample)

Discovery-based or Targeted GC-MS

  • Agilent 7200 accurate mass GC/QTOF-MS ($62.90/hr*)

Targeted LC-MS

  • SRM (selected reaction monitoring) on SCIEX QTRAP 5500, SCIEX QTRAP 6500+, Agilent 6495C
  • PRM or SIM on Thermo Q Exactive Orbitrap ($120/hr*)

* University of Minnesota Internal Customer rates

 

The rates shown below are for samples submitted to the CMSP on or after July 1, 2019.

 

UMN

Internal RATES

External

RATES

INSTRUMENTS

 

LC-MS
  
Orbitrap Fusion, Thermo (hourly, full service)
$95.00
$190.00
Orbitrap Velos, Thermo (hourly, full service)
$70.00
$140.00
LTQ, Thermo (hourly, full service) 
$50.00
$100.00
Orbitrap Q-Exactive, Thermo (hourly, full service)
$120.00
$240.00
LCQ, Thermo (hourly)
$49.50
$99.00
QTRAP 5500, SCIEX (hourly)
$110.00
$220.00
QTRAP 6500*, SCIEX (hourly, full service)
$130.00
$260.00
Triple Quadrupole 6495C, Agilent (hourly, fullservice)$130.00$260.00
Orbitrap Eclipse, Thermo (Hourly, full service)$130.00$260.00
GC-MS
  
7200 Quadrupole Time-of-Flight, Agilent (hourly, full service)
$62.90
$125.80
MALDI-TOF
 
 
BRUKER Autoflex (per sample, full service)
$48.50
$97.00
BRUKER Autoflex (per hour, trained user)
$80.25 
$160.50
SAMPLE PREPARATION
  
Method Development (hourly)
$97.56
$195.12
Sample Preparation (hourly)
$80.47
$160.94
Protein Quantification Assay (per set of samples)
$80.47
$160.94
Barocycler use and consumables (per sample)
 $15.20
$30.40
Off-line LC (C18 high pH) (per run)
$275.00
$550.00
Phosphorylation Enrichment, Fe-NTA and TiO2 column (Pierce) (per sample)
$179.40
$358.80
PROTEOLYTIC DIGESTIONS AND SDS-PAGE
  
SDS-PAGE gel analysis
$92.50
$185.00
Trypsin vial
$14.96
$29.92
Digestion in-gel (full service, 10 samples)
$165.00
$330.00
Digestion in-gel (cost per band after 10)
$13.00
$26.00
Trypsin digest in-solution (full service, per sample)                                         
$20.00
$40.00
Asp-N enzyme (add-on to trypsin digest)
$148.58
  $297.16
GluC enzyme (add-on to trypsin digest)
$50.28
$100.56
Chymotrypsin enzyme (add-on to trypsin digest)                                         
$42.94
$85.88
Trypsin/Lys-C enzyme (add-on to trypsin digest)
$25.84
$51.68
Lys-C enzyme (add-on to trypsin digest)
$119.43
$238.86
Pepsin enzyme (add-on to trypsin digest)
$1.00
$2.00
ISOTOPE LABELING
  
TMT 10plex™ (Thermo) reagent only - Phospho enrichment + Global analysis$1086.80$2173.60
TMT 10plex™ (Thermo) reagent only - Global analysis (no enrichment)
$562.50
$1125.00
iTRAQ® 8plex (SCIEX) reagent, labeling, sample prep
$767.80
$1535.60
iTRAQ® 4plex (SCIEX) reagent, labeling, sample prep
$625.90
$1251.80
SAMPLE CLEAN-UP
  
ZipTip (consumable only)
$3.00
$6.00
ZipTip (Millipore) (full service)
$25.00
$50.00
Stage Tip (C18 or MCX) (full service)
$13.16
$26.32
SPE Stage Tip (C18 or MCX) (consumable only)
$0.50
$1.00
SPE MCX Cartridge (full service)
$25.00
$50.00
SPE MW 3K cutoff (column only)
$3.30
$6.60
SPE C18 (full service)
$25.00
$50.00
SPE C18 1 cc (column only)
$1.56
$3.12
SPE C18 3 cc (column only)
$2.48
$4.96
SPE MCX 1 cc (column only)
$1.98
$3.96
SPE MCX 3 cc (column only)
$2.85
$5.70
DATA ANALYSIS
 
 
Data Analysis (hourly)
$116.14
$232.28