You are here

You are here

Osha Roopnarine

Research Assistant Professor

Expertise:

Research statement

My research goal is to study the molecular mechanism of muscle contraction, with an emphasis on hypertrophic cardiomyopathy (HCM) disease. I am using multidisciplinary approaches such as biochemistry (steady state and transient kinetics), molecular biology (site-directed mutagenesis of muscle proteins to introduce probes), muscle mechanics, and biophysics (spectroscopy) to solve fundamental problems in the mechanism of muscle contractility. I use fluorescence resonance energy transfer (FRET), and electron paramagnetic resonance (EPR) spectroscopies to study the myosin-actin interaction and the lever arm rotation in solution and in muscle fibers. I am also developing fluorescent myosin biosensors for high-throughput screening assays for small compounds. Collaborators: Dr. David D. Thomas, Dr. Piyali Guhathakurta


Recent/relevant publications

J. A., Rohde, O. Roopnarine, D. D. Thomas, J. M. Muretta. 2018. Mavacamten stabilizes an autoinhibited state of two-headed cardiac myosin. Proc Natl Acad Sci U S A.115(32):E7486-E7494. PMID: 30018063 
    
P. Guhathakurta, E. Prochniewicz, O. Roopnarine, J. A. Rohde, and D. D. Thomas. 2017. A Cardiomyopathy Mutation in the Myosin Essential Light Chain Alters the Structural Interaction with Actin. Biophys. J.113:91-100. PMID: 28700929 
    
O. Roopnarine. 2003. Mechanical defects of muscle fibers with myosin light chain mutants that cause cardiomyopathy. Biophys. J., 84:2440-2449. PMID: 12668451 
    
O. Roopnarine. 2002. Familial Hypertrophic Cardiomyopathic myosin mutations that affect the actin-myosin interaction. In Molecular Interactions of Actin. Actin-Myosin Interaction and Actin-Based Regulation. Springer-Verlag Berlin Heidelberg, Germany. Results Probl. Cell. Differ. 36:75-86. PMID: 11892286 
    
D. D. Thomas, E. Prochniewicz and O. Roopnarine. 2002. Changes in Actin and Myosin Structural Dynamics Due to Their Weak and Strong Interactions. In Molecular Interactions of Actin. Actin-Myosin Interaction and Actin-Based Regulation. Springer-Verlag Berlin Heidelberg, Germany. Results Probl. Cell. Differ. 36:7-19. PMID: 11892285 
    
D. D. Thomas and O. Roopnarine. 2002. An overview of the actomyosin interaction. In Molecular Interactions of Actin. Actin-Myosin Interaction and Actin-Based Regulation. Springer-Verlag Berlin Heidelberg, Germany. Results Probl. Cell. Differ. 36:1-5. 
    
O. Roopnarine and L.A. Leinwand. 1998. Functional analysis of myosin mutations that cause familial hypertrophic cardiomyopathy. Biophys. J., 75:3023-3030. PMID: 9826622 
    
O. Roopnarine, A.G. Szent-Györgyi, and D.D. Thomas. 1998. Microsecond rotational dynamics of spin-labeled myosin regulatory light chain induced by relaxation and contraction of scallop muscle. Biochemistry, 37:14428-14436. PMID: 9772169 
    
O. Roopnarine and D.D. Thomas. 1996. Orientational dynamics of intermediate nucleotide states of indane dione spin-labeled myosin heads in skeletal muscle fibers. Biophys. J., 70:2795-2806. PMID: 8744317 
    
O. Roopnarine and D.D. Thomas. 1995. Orientational dynamics of indane-dione spin-labeled myosin heads in relaxed and contracting skeletal muscle fibers. Biophys. J., 68:1461-1471. PMID: 7787032 
    
O. Roopnarine, A.G. Szent-Györgyi, and D.D. Thomas. 1995. Saturation transfer electron paramagnetic resonance of spin-labeled myosin regulatory light chains in contracting muscle fibers. Biophys. J. 68:337s 
    
Thomas, D.D., R. Ramachandran, O. Roopnarine, D.W. Hayden and E.M. Ostap. 1995. The mechanism of force generation in myosin, a disorder-to-order transition, coupled to internal structural changes. Biophys. J., 68:135s-141s. PMID: 7787056 

O. Roopnarine and D.D. Thomas. 1994. A spin label that binds to myosin heads in muscle fibers with its principal axis parallel to the fiber axis. Biophys. J., 67:1634-1645. PMID: 7819495 
    
O. Roopnarine, K. Hideg, and D.D. Thomas. 1993. Saturation Transfer EPR of an Indane-Dione Spin-Label: Calibration with Hemoglobin and Application to Myosin Rotational Dynamics. Biophys. J., 64:1896-1906. PMID: 8396449 
    
P. James, A.K. Alrich, O. Roopnarine and J. Parker. 1989. Context specific misreading of phenylalanine codons. Molecular General Genetics 218:397-401. PMID: 2685541 

P:
Office: 612-626-8470 Lab: 612-626-8470
E:

roopn001@umn.edu

5-121 NHH (Office)
312 Church Street SE
Minneapolis, MN 55455
5-271 NHH (Lab)
312 Church Street SE
Minneapolis, MN 55455