The Magee laboratory has been one of the leaders in characterizing the genome of the human commensal/pathogenic fungus, Candida albicans. They study the chromosome organization, chromosome dynamics, and the phenotypic consequences of these phenomena. They are also helping to match the emerging sequence of the related fungus, Candida dubliniensis, to the karyotype.
B.B. Magee has recently developed a mating system in this nominally asexual yeast and has shown that many of the genes involved in mating in Saccharomyces cerevisiae are also required > for mating in Candida. The alpha pheromone, a molecule involved in signaling between cells of different mating types, has been identified. A significant fraction of clinical isolates is homozygous for the mating type locus and is able to mate. The significance of mating in infection is under investigation.
Selected Publications (Pubmed Search)
Chibana, H,, N.Oka, H. Nakayama, T. Aoyama, B.B. Magee, P.T. Magee, and Y. Mikami. 2005. Sequence finishing and gene mapping for Candida albicans chromosome 7 and syntenic analysis against the Saccharomyces cerevisiae genome. Genetics: 170: 1525-1537. Ibrahim, A. S., B.B. Magee, D.C. Shephard, Molly Yang, Sarah Kaufman, Jeff Becker, John S. Edwards, jr, and P.T. Magee. 2005. Effect of ploidy and mating type on virulence in Candida albicans. Inf. Imm. 73: 7366-7374.
Lephart P, H Chibana, and PT Magee (2005). Effect of the major repeat sequence (MRS) on chromosome loss in Candida albicans. Eukarot Cell 4:733-741.
Forche A, G May, and PT Magee (2005). Demonstration of loss of heterozygosity by single-nucleotide polymorphism microaray analysis and alterations in strain morphology in Candida albicans strains during infection. Eukaryot Cell. 4: 156-65.
Panwar, S., M. Legrande, D. Dignard, M. Whiteway, and P.T. Magee. 2003. MFalpha1, the gene encoding the alpha mating pheromone of Candida albicans. Eukaryot Cell. 2:1350-60.
Xi Chen, B B Magee, Dean Dawson, PT Magee, Carol A, Kumamoto. 2004. Chromosome trisomy compromises the virulence of Candida albicans Mol. Microbiol.51:551-65.
Legrand, M. P. Lephart, A. Forche, F.M. Mueller, T. Walsh, P.T. Magee and B.B. Magee. 2004. Homozygosity at the MTL locus in Candida albicans: karyotypic rearrangements and tetraploid formation. Molecular Microbiol. 52: 1451-1462
Forche, A., P.T. Magee, B.B. Magee, and G. May.2004. Development of a genome-wide SNP map for Candida albicans. Eukaryot. Cell 3: 705-714
Jones, Ted, Nancy A. Federspiel , Hiroji Chibana, Jan Dungan, Sue Kalman, B. B. Magee, George Newport, Yvonne R. Thorstenson, Nina Agabian, P. T. Magee, Ronald W. Davis, and Stewart Scherer. 2004. The diploid genome sequence of Candida albicans. Proc. Nat. Acad. Sci. USA 101: 7329-34
Iwaguchi S, Suzuki M, Sakai N, Nakagawa Y, Magee PT, and Suzuki T. 2004 . Chromosome translocation induced by the insertion of the URA blaster into the major repeat sequence (MRS) in Candida albicans. Yeast. 21:619-34.
Forche A, May G, Magee PT. 2005. Demonstration of Loss of Heterozygosity by Single-Nucleotide Polymorphism Microarray Analysis and Alterations in Strain Morphology in Candida albicans Strains during Infection.Eukaryot Cell. 4:156-65
Magee PT, Magee BB. 2004.Through a glass opaquely: the biological significance of mating in Candida albicans.Curr Opin Microbiol. 7:661-5