A number of studies on patients who experienced heart failure have shown low calcium pump activity inside their cardiac muscle tissue. The membrane proteins which are primarily responsible for controlling Ca2+ concentration inside muscle cells are the ryanodine receptor (RyR), and the sarcoplasmic reticulum Ca2+-ATPase (SERCA). Upon binding to SERCA, the small membrane protein phospholamban (PLB) has been shown to greatly inhibit SERCA’s activity. However, SERCA activity can be partially restored upon phosphorylation of PLB or micromolar Ca2+ concentration in the cytosol. In order to better understand the structural mechanisms behind these phenomena, I employ electron paramagnetic resonance spectroscopy in conjunction with site-directed spin labeling on PLB and SERCA.
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M. A. Zudov, O. A. Mironov, Q. A. Ebner, P. D. Martin, Q. Shi, and D. R. Leadley, Observation of microwave-induced resistance oscillations in a high-mobility two-dimensional hole gas in a strained Ge/SiGe quantum well, Phys. Rev. B 89, 125401 (2014).