Polymerase Chain Reaction (PCR) For a 50 µl reaction, use:5 µl 10x PCR buffer7 µl 25 mM MgCl22 µl dNTP1 µl primer A1 µl primer B1 µl Taq polymerase1 µl template DNA32 µl ddH2O 10x PCR buffer:5 parts 1 M KCl1 part 1 M Tris, pH 8.04 parts ddH2O