cDNA library screening allows detection of expressed genes for subsequent cloning and sequencing.
Selecting a Library
Currently, our lab has access to cDNA libraries from three developmental time points.
- Maternal: messages from embryos before midblastula-transistion, when zygotic transcription begins (~0-3 hours of development).
- Shield: messages from the shield stage, which signifies the beginning of gastrulation (~6 hours of development).
- Post-somitegenesis: messages after the formation of somites is complete (~24 hours of development).
Choose the appropriate library for when you expect your message of interest is expressed.
Overview of Procedures