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Precipitating DNA

  1. Mix 40ul of PCR product + 80ul 100% EtOH + 4ul 3M NaOAc in 1.5 ml snap top tube. [1Vol + 2Vol 100% EtOH + 0.1Vol 3M NaOAc]
  2. Ice for 20 Minutes
  3. Spin 5 minutes, pour off
  4. Wash in ~140ul EtOH, spin 5 minutes
  5. Dry
  6. Resuspend in 25ul H2O