CMSP Proteomics Workshop Itinerary
August 9 – 11, 2016
Tuesday August 9, 2016
(Gortner Lab Room No. 239)
9:00 – 9:15 Welcome Pratik Jagtap (Gort 239)
9:15 - 10:00 Introduction to Proteomics I Tim Griffin (Gort 239)
10:00– 10:45 Introduction to Proteomics II Susan Van Riper (Gort 239)
10:45– 11:00 Break
11:00– 12:30 Sample Preparation Basics Todd Markowski (Gort 239)
12:30 – 1:30 Lunch Break
(Gortner Lab Rom No. 43)
1:30 – 4:00 Workshops Ia, Ib and Ic (Gort 43) (split into groups)*
Workshop Ia* Stage-tip and representative MALDI analysis Todd Markowski
Workshop Ib* Protein estimation methods Stephen Harvey
Workshop Ic* Sample Preparation Technologies and Instrumentation Bruce Withuhn
AUGUST 10, 2016 Wednesday
(Gortner Lab Room No. 239)
9:00 – 9:45 Introduction to Mass Spectrometry: Mass Spec 101Tom Krick (Gort 239)
9:50 – 11:15 Tandem MS and Intro to Liquid Chromatography-MS LeeAnn Higgins (Gort 239)
11:15 – 11:30 Break
11:30 – 12:30 Specialized Applications in mass spectrometry Bruce Withuhn (Gort 239)
12:30 – 1:30 Lunch Break
(Gortner Lab Rom No. 43)
1:30 – 4:00 Workshops IIa, IIb, IIc (Gort 43) (split into groups)*
(Take Breaks as necessary in between workstations)
Workshop IIa ESI-Ion Trap MS: Peptide MS/MS and intact protein (Gort 43) Tom Krick
Workshop IIb ESI-Orbitrap MS: Ultra high accuracy MS and MS/MS (Gort 43) LeeAnn Higgins
Workshop IIc Manual interpretation of MS/MS spectrum (Gort 43) Candace Guerrero
AUGUST 11, 2016 Thursday
(Gortner Lab Room No. 239)
9:00 – 9:50 ProteoInformatics Overview Pratik Jagtap (Gort 239)
9:50 - 10:00 Break
10:00 – 11:15 Protein Identification LeeAnn Higgins (Gort 239)
11:15 – 12:15 Protein Validation and Quantification Susan Van Riper (Gort 239)
12:15 – 12:30 Publishing Your Data Pratik Jagtap (Gort 239)
12:30 – 1:30 Lunch Break
1:30 – 4:30 ProteoInformatics Workshop (Cargill Building SCI 138 (computer lab))
1:30 to 2:30 1) MALDI-TOF Results: Peptide Mass Fingerprinting LeeAnn Higgins
2:30 to 3:30 2) Using Galaxy-P for MS/MS Search and related workflows Pratik Jagtap
3:30 to 4:30 3) Scaffold Review Susan van Riper