The ability to precisely alter DNA sequences in living cells makes possible detailed functional analysis of genes and genetic pathways. In plants, targeted genome modification has applications ranging from understanding plant gene function to developing crops with new traits of value. We have enabled efficient methods for targeted modification of plant genomes using sequence-specific nucleases. With zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs), and CRISPR/Cas9 reagents, we have achieved targeted gene knockouts, replacements and insertions in a variety of plant species. Current work is focused on optimizing delivery of nucleases and donor DNA molecules to plant cells to more efficiently achieve targeted genetic alterations.
Selected Publications: PubMed
Zsögön, A, Čermák, T, Naves, EE, Notini, MM, Edel, KH, Weinl, S, Freschi, L, Voytas, DF, Kudla, J, Peres, LEP. (2018) De novo domestication of wild tomato using genome editing. Nat Biotechnology. [Epub ahead of print]
Patrinostro X, Roy P, Lindsay A, Chamberlain CM, Sundby LJ, Starker CG, Voytas DF, Ervasti JM, Perrin BJ. (2018) Essential nucleotide- and protein-dependent functions of Actb/β-actin. Proc Natl Acad Sci U S A. 2018 Jul 31;115(31):7973-7978.
Shan, Q., Voytas, D.F. (2018) Editing plant genes one base at a time. Nature Plants. [Epub ahead of print]
Zhang, F., Voytas, D.F. (2018) Synthetic genomes engineered by SCRaMbLEing. SCIENCE CHINA Life Sciences.
Bhowmik P, Ellison E, Polley B, Bollina V, Kulkarni M, Ghanbarnia K, Song H, Gao C, Voytas DF, Kagale S. (2018) Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Sci Rep. 8(1):6502.
Macovei A, Sevilla NR, Cantos C, Jonson GB, Slamet-Loedin I, Čermák T, Voytas DF, Choi IR, Chadha-Mohanty P. (2018) Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus. Plant Biotechnol J. [Epub ahead of print]
He J, Xu M, Willmann MR, McCormick K, Hu T, Yang L, Starker CG, Voytas DF, Meyers BC, Poethig RS. (2018) Threshold-dependent repression of SPL gene expression by miR156/miR157 controls vegetative phase change in Arabidopsis thaliana. PLoS Genet. 14(4):e1007337. [Epub ahead of print]