The ability to precisely alter DNA sequences in living cells makes possible detailed functional analysis of genes and genetic pathways. In plants, targeted genome modification has applications ranging from understanding plant gene function to developing crops with new traits of value. We have enabled efficient methods for targeted modification of plant genomes using sequence-specific nucleases. With zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs), and CRISPR/Cas9 reagents, we have achieved targeted gene knockouts, replacements and insertions in a variety of plant species. Current work is focused on optimizing delivery of nucleases and donor DNA molecules to plant cells to more efficiently achieve targeted genetic alterations.
Selected Publications: PubMed Search
Nadakuduti SS, Starker CG, Voytas DF, Buell CR, Douches DS. (2019) Genome Editing in Potato with CRISPR/Cas9. Methods Mol Biol. 2019;1917:183-201.
Nadakuduti SS, Starker CG, Ko DK, Jayakody TB, Buell CR, Voytas DF, Douches DS. (2019) Evaluation of Methods to Assess in vivo Activity of Engineered Genome-Editing Nucleases in Protoplasts. Front Plant Sci. 10:110.
Bhowmik, P., Ellison, E., Polley, B., Bollina, V., Kulkarni, M., Ghanbarnia, K., Song, H., Gao, C. X., Voytas, D. F. and Kagale, S. (2018) Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Scientific Reports, 8.
Curtin, S. J., Xiong, Y., Michno, J. M., Campbell, B. W., Stec, A. O., Cermak, T., Starker, C., Voytas, D. F., Eamens, A. L. and Stupar, R. M. (2018) CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula. Plant Biotechnology Journal, 16(6), pp. 1125-1137.
He, J., Xu, M. L., Willmann, M. R., McCormick, K., Hu, T. Q., Yang, L., Starker, C. G., Voytas, D. F., Meyers, B. C. and Poethig, R. S. (2018) Threshold-dependent repression of SPL gene expression by miR156/miR157 controls vegetative phase change in Arabidopsis thaliana. Plos Genetics, 14(4).
Hummel, A. W., Chauhan, R. D., Cermak, T., Mutka, A. M., Vijayaraghavan, A., Boyher, A., Starker, C. G., Bart, R., Voytas, D. F. and Taylor, N. J. (2018) Allele exchange at the EPSPS locus confers glyphosate tolerance in cassava. Plant Biotechnology Journal, 16(7), pp. 1275-1282.
Macovei, A., Sevilla, N. R., Cantos, C., Jonson, G. B., Slamet-Loedin, I., Cermak, T., Voytas, D. F., Choi, I. R. and Chadha-Mohanty, P. (2018) Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus. Plant Biotechnology Journal, 16(11), pp. 1918-1927.
Nadakuduti, S. S., Buell, C. R., Voytas, D. F., Starker, C. G. and Douches, D. S. (2018) Genome Editing for Crop Improvement - Applications in Clonally Propagated Polyploids With a Focus on Potato (Solanum tuberosum L.). Frontiers in Plant Science, 9.
Patrinostro, X., Roy, P., Lindsay, A., Chamberlain, C. M., Sundby, L. J., Starker, C. G., Voytas, D. F., Ervasti, J. M. and Perrin, B. J. (2018) Essential nucleotide- and protein-dependent functions of Actb/beta-actin. Proceedings of the National Academy of Sciences of the United States of America, 115(31), pp. 7973-7978.
Sanchez-Leon, S., Gil-Humanes, J., Ozuna, C. V., Gimenez, M. J., Sousa, C., Voytas, D. F. and Barro, F. (2018) Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9. Plant Biotechnology Journal, 16(4), pp. 902-910.
Shan, Q., Baltes, N. J., Atkins, P., Kirkland, E. R., Zhang, Y., Baller, J.A., Lowder, L. G., Malzahn, A. A., Haugner, J. C., 3rd, Seelig, B., Voytas, D. F. and Qi, Y. (2018) ZFN, TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis: A disconnect between somatic and germinal cells. J Genet Genomics.
Shan, Q. W. and Voytas, D. F. (2018) Editing plant genes one base at a time A new class of gene-editing reagents precisely alters plant genomes without creating a DNA double strand break. Nature Plants, 4(7), pp. 412-413.
Zhang, F. and Voytas, D. F. (2018) Synthetic genomes engineered by SCRaMbLEing. Science China-Life Sciences, 61(8), pp. 975-977.