Physiology and Synthetic Biology of Environmental Bacteria
Our lab is interested in understanding the physiology of Shewanella, a species of gram-negative bacteria found throughout the world in aquatic environments. This group has the greatest diversity of compounds it can ‘breathe’ of any organisms studied to date. The act of respiring these compounds can impact geochemical gradients of compounds like sulfur, iron and manganese. The molecular mechanism that enables S. oneidensis to carry out these reactions and how these systems are regulated is a main focus of research in my laboratory. By understanding the mechanistic details of these processes we can begin to rationally engineer S. oneidensis (and its relatives) for biotechnological applications, ranging from bioremediation to energy generation to biocatalysis.
Bennett B.D., K.E. Redford and J.A. Gralnick. 2018. Survival of anaerobic Fe2+ stress requires the ClpXP protease. J Bacteriol. 200(8):e00671-17 doi: 10.1128/JB.00671-17.
Bennett, B.D., K.E. Redford and J.A. Gralnick. 2018. The MgtE homolog FicI acts as a secondary ferrous iron importer in Shewanella oneidensis strain MR-1. Appl Environ Microbiol. 84(6):e01245-17 doi: 10.1128/AEM0.1245-17.
Rowe, A.R., P. Rajeev, A. Jain, S. Pirbadian, A. Okamoto, J.A. Gralnick, M.Y. El-Naggar and K.H. Nealson. 2018. Tracking electron uptake from a cathode into Shewanella cells: implications for energy acquisition from solid-substrate electron donors. 9(1):e02203-17. doi: 10.1128/mBio.02203-17
West, E.A., A. Jain and J.A. Gralnick. 2017. Engineering a native inducible expression system in Shewanella oneidensis to control extracellular electron transfer. ACS Synth Biol. doi: 10.1021/acssynbio.6b00349