I am involved with collaborative projects, headed by my colleagues in the Center for Genome Engineering, the UMN Stem Cell Institute, and the Masonic Cancer Center at UMN, that focus on gene therapy and genome editing in animals. For gene therapy we are improving the delivery and placement of Sleeping Beauty transposons in order to improve their use as non-viral vectors. We use site-specific nucleases for precision genetic engineering (genome editing) to introduce specific sequences into animal genomes. We also use site-specific nucleases to engineer large animals to be hosts for human iPSC-derived tissues.
Selected Publications: PubMed Search
Kebriae, P., Singh H., Huls, M.H., Figliola, M.J., Bassett, R., Olivares, S., Ang, S., Dawson, M.J., Kumaresan, P.R., Su, S., Maiti, S., Dai, J., Moriarity, B., Forget, M.A., Senyukov, V., Orozco, A., Liu, T., McCarty, J., Jackson, R.N., MoyesJ.S., Rondon, G., Qazilbash, M., Ciurea, S., Alousi, A., Nieto, Y., Rezvani, K., Marin, D., Popat, U., Hosing, C., Shpall, E.J., Kantarjian, H., Keating, M.,Wierda, M., Do, K.A., Largaespada, D.A., Lee, D.A., Hackett, P.B., Champlin, R.E., and Cooper, L.J.N. (2016) Phase I trials using Sleeping Beauty to generate CD19-specific T cells. J. Clin. Invest. 126: 3363-3376 Watson, A.L., Carlson, D.F., Largaespada, D.A., Hackett, P.B., and Fahrenkrug, S.C. (2016). Engineered swine models of cancer. In Frontiers in Genetics, Cancer Genetics. 7: 78
Hyland, K.A., Aronovich, E.L., Olson, E.R., Bell, J.B., Urness, M., Gunther, R., Hunter, D.W., Hackett, P.B. and McIvor, R.S. (2017). Transgene expression in dogs after liver-directed hydrodynamic delivery of Sleeping Beauty transposons using balloon catheters. Hum. Gene Ther. 28: 541-550 [PMID: 28447859]
Aronovich, E.L., Hyland, K.A., Olson, E.R., Bell, J.B., Urness, M., Gunther, R., Hunter, D.W., McIvor, R.S. and Hackett, P.B. (2017). Treatment of a-L-iduronidase deficiency in dogs using the Sleeping Beauty transposon system. Hum. Gene Ther. 28: 551-564 [PMID: 28530135]
Fong, Y., Hackett, P.B. (2017). Acceptance and Access to Gene Editing: Science and Our Obligations to Mankind. Mol. Ther. 25: 1-2
Phillips, H.R., Tolstyka, Z.P., Hall, B.C., Hexum, J. Hackett, P.B., Reineke, T.R. (2019). Glycopolycation-DNA polyplexes formulation N/P ratio affects stability, hemocompatibility, and in vivo biodistribution. Biomacromolecules 20: 1530-1544.