Ph.D., Biochemistry, Molecular Biology and Biophysics, 1997; University of Minnesota, St. Paul, MN

Postdoctoral Associate

University of Minnesota, St. Paul, MN, Department of Biochemistry, Molecular Biology & Biophysics, 1997-2000

Research Associate

University of Minnesota St. Paul, MN, Department of Biochemistry, Molecular Biology & Biophysics, 2000-2002

Research Assistant Professor

University of Minnesota St. Paul, MN, Department of Biochemistry, Molecular Biology & Biophysics, 2002-2003

Research Scientist II

Department of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 2004

Research Assistant Professor

University of Minnesota, St. Paul, MN, Department of Biochemistry, Molecular Biology & Biophysics, 2004-present

Expertise

Protein labeling protocol iTRAQ

HPLC and FPLC separations

Affinity chromatography

Protein purification and prep

Membrane protein purification

Bacterial culture

Peptide and small molecule ESI mass spectrometry

Protein identification and amino acid PTM assignments by tandem MS interpretation

Relative abundance protein quantification by iTRAQ technology

Absolute quantification of peptides and small molecules using MRM mass spectrometry

Awards

2000-2001 Recipient of Paul D. Boyer Award

Publications

Kelly R. F., Cabrera, J. A., Ziemba, E. A., Crampton, M., Anderson, L. B., McFalls, E.O., and Ward HB. (2011) Continued depression of maximal oxygen consumption and mitochondrial proteomic expression despite successful coronary artery bypass grafting in a swine model of hibernation. J Thorac Cardiovasc Surg. 141(1):261-8

Serrano J, Higgins L, Witthuhn BA, Anderson LB, Markowski T, Holcombe GW, Kosian PA, Korte JJ, Tietge JE, Degitz SJ. (2010) In vivo assessment and potential diagnosis of xenobiotics that perturb the thyroid pathway: Proteomic analysis of Xenopus laevis brain tissue following exposure to model T4 inhibitors. Comp Biochem Physiol Part D Genomics Proteomics. Jun;5(2):138-50.

Boylan KL, Andersen JD, Anderson LB, Higgins L, Skubitz AP. (2010) Quantitative proteomic analysis by iTRAQ(R) for the identification of candidate biomarkers in ovarian cancer serum. Proteome Sci. 14;8:31

Zhang, J., Wang, L., Anderson, L. B., Witthuhn, B., Xu, Y., and L. J. (2010) Proteomic profiling of potential molecular targets of methyl-selenium compounds in the transgenic adenocarcinoma of mouse prostate model. Cancer Prev Res (Phila).3(8):994-1006

Salinas, K., Hemmer, M.J., Serrano, J., Higgins, L., Anderson, L. B., Benninghoff, A. D., Williams, D. E., and Walker C. (2010) Identification of estrogen-responsive vitelline envelope protein fragments from rainbow trout (Oncorhynchus mykiss) plasma using mass spectrometry. Mol Reprod Dev. 77(11):963-70

Anderson JD, Boylan KL, Xue FS, Anderson LB, Witthuhn BA, Markowski TW, Higgins L, and Skubitz AP, (2010) Identification of candidate biomarkers in ovarian cancer serum by depletion of highly abundant proteins and differential in-gel electrophoresis. Feb 16;31(4):599-610. [Epub ahead of print]

Boyce M, Malone ED, Anderson LB, Park S, Godden SM, Jenner F, and Trumbel TN, (2010), Am J Vet Res. Feb;71(2):169-75.

Riedl MS, Braun PD, Kitto KF, Roiko SA, Anderson LB, Honda CN, Fairbanks CA, and Vulchanova L. (2009), Proteomic analysis uncovers novel action of the neurosecretory protein VGF in nociceptve processing. J Neurosci. Oct 21;29(42):13377-88.

Canales BK, Anderson L, Higgins L, Frethem C, Ressler A, Kim IW, and Monga M, (2009) Proteomic analysis of a matrix stone: a case report, Urol Res., Sep 4. [Epub ahead of print]

Canales BK, Higgins L, Markowski T, Anderson L, Li QA, and Monga M, (2009) Presence of 5 conditioning film proteins are highly associated with early stent encrustation, J Endourol. /23(9):1437-42

Canales BK, Anderson L, Higgins L, Slaton J, Roberts KP, Liu N, and Monga M, (2008) Second Prize: Comprehensive analysis of human calcium oxalate monohydrate kidney stone matrix. J Endourol. 22(6) 1161-7

Kassie, F., Anderson, L.B., Higgins, L., Pan, Y., Matise, I., Negia, M., Upadhyaya, P., Wang, M. and Hech, S. S., (2008) Chemopreventive agents modulate the protein expression profile of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone plus benzo[a]pyrene-induced lung tumors in A/J mice. Carcinogenesis, 29:610-619

Kassie, F., Anderson, L. B., Scherber, R., Yu, N., Lahti, D., Upadhyaya, P., and Hecht, S. S., (2007) Indole-3-carbinol Inhibits 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone Plus Benzo[a]pyrene-Induced Lung Tumorigenesis in A/J Mice and Modulates Carcinogen-Induced Alterations in Protein Levels. Cancer Research, 67:6502-6511

Lund, T.C., Anderson L.B., McCullar, V., Higgins, L., Yun, G.H., Grzywacz, B., Verneris, M.R., Miller, J.S. (2007) iTRAQ is a useful method to screen for membrane-bound proteins differentially expressed in human natural killer cell types. J. Proteome Res. 6(2):644-53

Anderson, L.B., Ouellette, A.J., Eaton-Rye,J., Maderia, M., MacCoss, M.J., Yates, J.R. III & Barry, B. A. (2004) Evidence for a post-translational modification, aspartyl aldehyde, in a photosynthetic membrane protein. Journal of the American Chemical Society, 126:8399-405

Anderson, L.B., Maderia, M., Ouellette, A.J.A., Putnam-Evans, C., Higgins, L., Krick, T., MacCoss, M.J., Lim, H., Yates, J. R. III, & Barry, B. A. (2002) Post-translational modifications in the CP43 Subunit of the Photosystem II. Proceedings of the National Academy of Sciences, USA, 99:14676-14681

Anderson, L.B., Ouellette, A.J.A., & Barry, B.A. (2000) Probing the structure of photosystem II using amines and phenylhydrazine. Journal of Biological Chemistry, 275:4920-4927

Ouellette, A. J. A., Anderson, L.B., & Barry, B.A. (1998) Amine binding and oxidation at thecatalytic site for photosynthetic water oxidation. Proceedings of the National Academy of Science, USA, 95:2204-2209

Das, A., Anderson, L.B., & Xie, Y-H. (1997) Delineation of the interaction domains of Agrobacterium tumefaciens VirB7 and VirB9 by the yeast two hybrid assay, Journal of Bacteriology. 179:3404-3409

Anderson, L.B., Hertzel, A.V., & Das, A. (1996) Agrobacterium tumefaciens VirB7 and VirB9 Form a Disulfide-Linked Protein Complex. Proceedings of the National Academy of Sciences, USA, 93:8889-8894