420 Washington Avenue SE
Minneapolis, MN 55455
We develop genetic engineering and genome-editing methods for biomedical applications. We focus on adapting the Sleeping Beauty transposon system (created in the lab in 1997) as a non-viral vector for human gene therapy. We use genome editing to support growing human replacement tissues and cells in large animals.
I am involved with collaborative projects, headed by my colleagues in the Center for Genome Engineering, the UMN Stem Cell Institute, and the Masonic Cancer Center at UMN, that focus on non-viral gene therapy using the Sleeping Beauty transposon system precision genetic engineering (genome editing) in large animals for improving animal welfare and quality, swine models of diseases, and regenerative medicine.
Hackett, P.B. (2020). Regulatory issues for genetically modified animals. Frontiers of Agricultural Science and Engineering 7(2): 188-203.
Crane, A. T., R. N. Aravalli, A. Asakura , D. Carlson, M. Cheeran, G. Danczyk, J. Dutton, A. W. Grande, P. B. Hackett, W. S. Hu, P. Igarashi, W. C. Lu, Z. Miller, T. O’Brien, A. Panosakaltsis-Mortari, A. M. Parr, C. Pearce, M. Ruiz-Estevez, M. Shiao, C. Sipe, N. K. Toman, J. Voth, H. Xie, C. J. Steer, and W. C. Low. 2019. Exogenesis of human tissues and organs for transplantation. Cell Transplant. 28: 1091-1105.
Gu, Q., X. Yang, Q. Li, Y. Long, G. Song, D. Pei, J. Chen, H. Peng, P. B. Hackett, and Z. Cui. 2019. Asymmetrical cleavages of Sleeping Beauty transposons generate multiple excised transposon fragments during transposition. bioRxiv 659086;
Fong, Y., P. B. Hackett. 2017. Acceptance and Access to Gene Editing: Science and Our Obligations to Mankind. Mol. Ther. 25: 1-2
Aronovich, E.L., K. A. Hyland, E. R. Olson, J. B. Bell, M. Urness, R. Gunther, D. W. Hunter, R. S. McIvor, P. B. and Hackett. 2017. Treatment of a-L-iduronidase deficiency in dogs using the Sleeping Beauty transposon system. Hum. Gene Ther. 28: 551-564
Hyland, K.A., E. L. Aronovich, E. R. Olson, J. B. Bell, M. Urness, R. Gunther, D. W. Hunter, P. B. Hackett, and R. S. McIvor. 201). Transgene expression in dogs after liver-directed hydrodynamic delivery of Sleeping Beauty transposons using balloon catheters. Hum. Gene Ther. 28: 541-550
Watson, A.L., D. F. Carlson, D. A. Largaespada, P. B. Hackett, and S. C. Fahrenkrug, S.C. 2016. Engineered swine models of cancer. Frontiers in Genetics, Cancer Genetics. 7: 78
Ph.D.: University of Colorado, 1974