Denhardt's Solution 100X
- 10g Ficoll 400
- 10g Polyvinylpyrrolidone
- 10g Bovine Serum Albumin
- H2O to 500 mL
20X SSC
- 175.3g NaCl
- 88.2g Sodium Citrate
- pH to ~7.0 with 10M NaOH
- Adjust volume to 1L
TE
- 10mM Tris (pH 7.4) (pH can be varied)
- 1mM EDTA (pH 8.0)