LB broth, agar
- 10g Tryptone
- 5g Yeast Extract
- 5g NaCl
- 100µL of 10 M NaOH
- H2O to 1L
- For agar add 15g of bacto-agar
- Autoclave to sterilize
NZY broth, agar, top agarose
- 5g NaCl
- 2g MgSO4*7H2O
- 5g Yeast Extract
- 10g NZ Amine-A (casein hydrolysate)
- 300µL of 10 M NaOH
- H2O to 1L
- For agar add 15g of bacto-agar
- For top agarose add 7g of agarose
- Autoclave to sterilize
NZYM broth, agar, top agarose
- Prepare NZY broth, agar, or top agarose.
- After autoclaving, add 50 mL of 20% Maltose per Liter of NZY
- Autoclaving can oxidize maltose and other sacharides. Therefore, the 20% maltose stock solution must be filter sterilized to prevent contamination.