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Protocols

 

Close-up shot of mushrooms

DNA extraction

REDE extraction: tips & tissue (.doc)
DNeasy extraction protocol for Frankia (.doc)
Short CTAB extraction protocol for general extractions (.doc)
CTAB extraction protocol for bulk root samples (.docx)

Media & Reagents

Agarose (.doc)
Blue juice loading dye (.doc)
DNA ladder (.doc)
Modified Melin-Norkrans (MMN) (.doc)
SB Buffer for gel loading (.txt)
2% CTAB buffer (.docx)

PCR, RFLP, and Sequencing

nifH PCR (for Frankia) (.doc)
ITS PCR (for fungi) (.doc)
Gel electrophoresis (.doc)
RFLP digests (.doc)
Sanger DNA sequencing (including PCR clean-up) (.doc)
Host plant ID - PCR (.doc)
Host plant ID - RFLP (.docx)

Bioassays & Determining mycorrhizal colonization

The Complete Guide (.doc)

Seed Germination

Alnus and Betula species (and Frankia) (.doc)
Pseudotsuga and other conifers (.doc)

 

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