Fungal High Throughput Amplicon Sequencing

Illumina sequencing & analysis protocol (.docx)
Illumina barcoded primers (.xlsx)
Illumina PCR dilutions & normalization (.xlsx)

Community discussion of fungal HTS methods (.gdoc)

DNA extraction

REDE extraction: tips & tissue (.doc)
DNeasy extraction protocol for Frankia (.doc)
Short CTAB extraction protocol for general extractions (.doc)
CTAB extraction protocol for bulk root samples (.docx)

Enzyme Activity Assays

Enzymatic activity assay for ectomycorrhizal root tips (.doc)

Media, Solutions, and more

Agarose (.doc)
Blue juice loading dye (.doc)
DNA ladder (.doc)
Modified Melin-Norkrans (MMN) (.doc)
SB Buffer for gel loading (.txt)
2% CTAB buffer (.docx)

PCR, RFLP, Sequencing, and more

nifH PCR (for Frankia) (.doc)
ITS PCR (for fungi) (.doc)
Gel electrophoresis (.doc)
RFLP digests (.doc)
Sanger DNA sequencing (including PCR clean-up) (.doc)
Host plant ID - PCR (.doc)
Host plant ID - RFLP (.docx)

Bioassay harvesting and scoring colonization

The Complete Guide (.doc)

Seed germination

Arbutus menziesii (Pacific madrone) (.doc)
Alnus and Betula species (and Frankia) (.doc)
Pseudotsuga and other conifers (.doc)