DNA extraction
REDE extraction: tips & tissue (.doc)
DNeasy extraction protocol for Frankia (.doc)
Short CTAB extraction protocol for general extractions (.doc)
CTAB extraction protocol for bulk root samples (.docx)
Media & Reagents
Agarose (.doc)
Blue juice loading dye (.doc)
DNA ladder (.doc)
Modified Melin-Norkrans (MMN) (.doc)
SB Buffer for gel loading (.txt)
2% CTAB buffer (.docx)
PCR, RFLP, and Sequencing
nifH PCR (for Frankia) (.doc)
ITS PCR (for fungi) (.doc)
Gel electrophoresis (.doc)
RFLP digests (.doc)
Sanger DNA sequencing (including PCR clean-up) (.doc)
Host plant ID - PCR (.doc)
Host plant ID - RFLP (.docx)
Bioassays & Determining mycorrhizal colonization
The Complete Guide (.doc)
Seed Germination
Alnus and Betula species (and Frankia) (.doc)
Pseudotsuga and other conifers (.doc)