Seelig, B. & Chen, I. A. (2024) Intellectual frameworks to understand complex biochemical systems at the origin of life Nature Chem. accepted
Lee, K. H., Distefano, M.D. & Seelig, B. (2023) Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl derivatized Sepharose 4B. Results Chem. PMID: (PDF) (Pubmed)
Zhu, B., Du, Z., Dai, Y., Kitaguchi, T., Behrens, S. & Seelig, B. (2023) Nanodroplet-based reagent delivery into water-in-fluorinated-oil droplets. Biosensors PMID: (PDF) (Pubmed)
Medina-Carmona, E., Gutierrez-Rus, L., Manssour-Triedo, F., Newton, M. S., Gamiz-Arco, G., Mota, A. J., Cuerva, J. M., Andrés-León, E., Ortega-Roldan, J. L., Seelig, B., Ibarra-Molero, B., Sanchez-Ruiz, J. M. (2023) Cell survival enabled by leakage of a labile metabolic intermediate. Mol. Biol. Evol. PMID: 36788592 (PDF) (Pubmed)
Tong, C. L. Kanwar, N, Morrone, D., & Seelig, B. (2022) Nature-inspired engineering of an artificial ligase enzyme by domain fusion. Nucleic Acids Res. (19) 11175. PMID: 36243966 (PDF) (Pubmed)
Kanwar, N., Blanco, C., Chen, I. A. & Seelig, B. PacBio sequencing output increased 5-fold through uniform and directional concatenation. Sci. Rep. 2021 (11) 18065 PMID: 34508117 (PDF) (Pubmed) In the News: (genomeweb)
Gamiz-Arco, G., Gutierrez-Rus, L.I., Risso, V.A., Ibarra-Molero, B., Hoshino, Y., Petrovic, D., Justicia, J., Cuerva, J.M., Romero-Rivera, A., Seelig, B., Gavira, J.A., Kamerlin, S.C.L., Gaucher, E.A., Sanchez-Ruiz, J.M. Novel heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase. Nature Commun. 2021 PMID (PDF) (Pubmed) In the News: (Human Frontier Science Program)
Tong, C. L., Lee, K.-H. & Seelig B. De novo proteins from random sequences through in vitro evolution. Curr. Opin. Struct. Biol. 2021 (68), 129-134. PMID: (PDF) (Pubmed)
Newton, M. S., Cabezas-Perusse, Y., Tong, C. L. & Seelig, B. In vitro selection of peptides and proteins - advantages of mRNA display. ACS Synth. Biol. 2020 (9), 181-190. PMID: 31891492 (PDF) (Pubmed)
Blanco, C., Verbanic, S., Seelig, B. & Chen, I. EasyDIVER: a pipeline for assembling and counting high throughput sequencing data from in vitro evolution of nucleic acids or peptides. Bioinformatics. J. Mol. Evol. 2020 (88), 477-481. PMID: 32529275 (PDF) (Pubmed)
Blanco, C., Verbanic, S., Seelig, B. & Chen, I. High throughput sequencing of in vitro selections for proteins using mRNA display. Phys. Chem. Chem. Phys. 2020 PMID: 31967131 (PDF) (Pubmed)
Newton, M. S., Morrone, D., Lee, K.-H. & Seelig, B. Genetic code evolution investigated through synthesis and characterisation of proteins from reduced alphabet libraries. ChemBioChem. 2019 (20) 846-856. (PDF) (Pubmed)
Shan, Q., Baltes, N. J., Atkins, P., Kirkland, E. R., Zhang, Y., Baller, J. A., Lowder, L. G., Malzahn, A. A., Haugner, J. C., Seelig, B., Voytas, D. F., Qi, Y. ZFN, TALEN and CRISPR-Cas9 mediated homology directed gene insertion in Arabidopsis: a disconnect between somatic and germinal cells. J. Genet. Genomics. 2018 (18) 30173-5. PMID 30598393 (PDF) (Pubmed)
Seelig, B. Multifunctional enzymes from reduced genomes - model proteins for simple primordial metabolism Molecular Microbiol. 2017 (105), 505-7. PMID 8665040 (PDF) (Pubmed)
Morelli, A., Cabezas, Y., Mills, L. J. & Seelig, B.: Extensive libraries of gene truncation variants generated by in vitro transposition. Nucleic Acids Res. 2017 (45) e78 (PDF) (Pubmed)
Lane M. D., & Seelig, B.: Highly efficient recombinant production and purification of streptococcal cysteine protease streptopain with increased enzymatic activity. Protein Expr. Purif. 2016 (121) 66-72. (>PDF) (Pubmed)
Lane M. D., Seelig B.: Advances in the directed evolution of proteins. Curr. Opin. Chem. Biol. 2014 (22) 129-136. (PDF) (Pubmed)
Morelli, A., Haugner III, J. C. & Seelig, B.: Thermostable artificial enzyme isolated by in vitro selection. PLOS ONE 2014 9(11): e112028. (PDF) (Pubmed)
Chao, F.-A., Morelli, A., Haugner III, J. C., Churchfield, L., Hagmann, L. N., Shi, L., Masterson, L. R., Sarangi, R., Veglia, G. & Seelig, B.: Structure and dynamics of a primordial catalytic fold generated by in vitro evolution. Nature Chem. Biol. 2013 (9) 81-83. (PDF) (NCB website)
In the News: (ACS Cent Sci) (Research Highlight - NASA) (Science Highlight - Stanford Synchrotron) (CBS Highlight) (SciTechDaily) (ScienceDaily) (Minnesota Daily) (reddit) (most read article of Nature Chem. Biol. !)
Golynskiy, M. V., Haugner III, J. C. & Seelig, B.: Highly diverse protein library based on the ubiquitous (β/α)8 enzyme fold yields well-structured proteins through in vitro folding selection. ChemBioChem 2013 (14) 1553-1563. (PDF) (Pubmed)
Haugner III, J. C. & Seelig, B.: Universal labeling of 5’-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity. Chem. Commun. 2013 (49) 7322-7324. (PDF) (Recommended by F1000Prime) (Pubmed)
Golynskiy, M. V., Haugner III, J. C., Morelli, A., Morrone, D., & Seelig, B.: In vitro evolution of enzymes. Meth. Mol. Biol. 2013 (978) 73-92. (PDF) (Pubmed)
Traaseth, N. J., Chao, F.-A., Masterson, L. R., Mangia, S., Garwood, M., Michaeli, S., Seelig, B., Veglia, G.: Heteronuclear Adiabatic Relaxation Dispersion (HARD) for Quantitative Analysis of Conformational Dynamics in Proteins. J. Magn. Reson. 2012 (219) 75-82. (PDF) (Pubmed)
Seelig, B.: mRNA display for the selection and evolution of enzymes from in vitro-translated protein libraries. Nature Protocols. 2011 (6) 540-552. (PDF)
Golynskiy, M. V. & Seelig, B.: De novo enzymes – from computational design to mRNA Display. Trends Biotechnol. 2010 (27) 340-345. (On the Cover & PDF)
Seelig, B.: An autocatalytic network for ribozyme self-construction. Nature Chem. Biol. 2008 (4) 654-655. (News & Views)
Seelig, B. & Szostak, J.W.: Selection and evolution of enzymes from a partially randomized non-catalytic scaffold. Nature, 2007 (44) 828-831. (PDF) (Supplementary Information)
In the News: (News & Views in Nature) (Spotlight in ACS Chem. Biol.) (Highlight in Nature Chem. Biol.) (Chemical & Engineering News)
Keiper, S., Bebenroth, D., Seelig, B., Westhof, E. & Jäschke, A.: An architecture of a Diels-Alder ribozyme with a preformed catalytic pocket. Chem. Biol. 2004 (11) 1217-1227. (PDF)
Keefe, A.D., Wilson, D.S., Seelig, B. & Szostak, J.W.: One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-tag. Protein Expr. Purif. 2001 (23) 440-446. (PDF) (Paper was top 5 downloaded article for the journal in 2002.)
Jäschke, A. & Seelig, B.: Evolution of DNA/RNA as catalysts for chemical reaction. Curr. Opin. Chem. Biol. 2000 (4) 257-262. (PDF)
Seelig, B., Keiper, S., Stuhlmann, F. & Jäschke, A.: Enantioselective ribozyme catalysis of a bimolecular cycloaddition reaction. Angew. Chem. Int. Ed. 2000 (39) 4576-4579. (PDF)
Sengle, G., Jenne, A., Arora, P. S., Seelig, B., Nowick, J. S., Jäschke, A. & Famulok, M.: Synthesis, incorporation efficiency, and stability of disulfide bridged functional groups at RNA 5'-Ends. Bioorg. Med. Chem. 2000 (8) 1317-1329. (PDF)
Seelig, B. & Jäschke, A.: Ternary conjugates of guanosine monophosphate as initiator nucleotides for the enzymatic synthesis of 5'-modified RNAs. Bioconjugate Chem. 1999 (10) 371-378. (PDF)
Seelig, B. & Jäschke, A.: A small catalytic RNA motif with Diels-Alderase activity. Chem. Biol. 1999 (6) 167-176. (PDF)
Seelig, B. & Jäschke, A.: Site-specific modification of enzymatically synthesized RNA: transcription initiation and Diels-Alder reaction.Tetrahedron Lett. 1997 (38) 7729-7732. (PDF)