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Gralnick Lab Publications


Conley, B.E., M.T. Weinstock, D.R. Bond and J.A. Gralnick. 2020. A hybrid extracellular electron transfer pathway enhances survival of Vibrio natriegens. App Environ Microbiol. 86(19):e01253-20. doi: 10.1128/AEM.01253-20.

Shaw D.R., A. Muhammad, K.P. Katuri, J.A. Gralnick, J. Reimann, R. Mesman, L. van Niftrik, M.S.M. Jetten and P.E. Saikaly. 2020. Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria. Nat Commun. 11(1):2058. doi: 10.1038/s41467-020-16016-y.

Jain, A. and J.A. Gralnick. 2020. Evidence for auxiliary anaerobic metabolism in obligately aerobic Zetaproteobacteria. ISME J. doi: 10.1038/s41396-020-0586-6.


Nisbett, L.M., L. Binnenkade, B. Bacon, S. Hossain, N.J. Kotloski, E.D. Brutinel, R. Hartmann, K. Drescher, D.P. Arora, S. Muralidharan, K.M. Thormann, J.A. Gralnick and E.M. Boon. 2019. NosP signaling modulates the NO/H-NOX-mediated multicomponent c-Di-GMP network and biofilm formation in Shewanella oneidensis. Biochemistry. 58(48):4827-4841. doi: 10/1021/acs.biochem.9b00706.

Chadwick, G.L., F. Otero Jiménez, J.A. Gralnick, D.R. Bond and V.J. Orphan. 2019. NanoSIMS imaging reveals metabolic stratification within current-producing biofilms. Proc Natl Acad Sci USA. pil: 21912498. doi: 10.1073/pnas.1912498116.

Kees, E.D., A.R. Pendleton, C.M. Paquete, M.B. Arriola, A.L. Kane, N.J. Kotloski, P.J. Intile and J.A. Gralnick. 2019. Secreted flavin cofactors for anaerobic respiration of fumarate and urocanate by Shewanella oneidensis: cost and role. Appl Environ Microbiol. pil: AEM.00852-19. doi: 10.112/AEM.00852-19.

Corts, A.D., L.C. Thomason, R.T. Gill and J.A. Gralnick. 2019. Efficient and precise genome editing in Shewanella with recombineering and CRISPR/Cas9-mediated counter-selection. ACS Synth Biol. 8(8):1877-1889. doi: 10.1021/acssynbio.9b00188.

Bennett, B.D. and J.A. Gralnick. 2019. Mechanisms of toxicity by and resistance to ferrous iron in anaerobic systems. Free Radic Biol Med. pil: S0891-5849(18)32165-8. doi: 10.1016/j.freeradbiomed.2019.06.027. 

Kaplan, M., P. Subramanian, D. Ghosal, C.M. Oikonomou, S. Pirbadian, R. Starwalt-Lee, S.K. Mageswaran, D.R. Ortega, J.A. Gralnick, M.Y. El-Naggar and G.J. Jensen. 2019. In situ imaging of the bacterial flagellar motor disassembly and assembly process. EMBO J. pil: e100957. doi: 10.15252/embj.2018100957.

Corts, A.D., L.C. Thomason, R.T. Gill and J.A. Gralnick. 2019. A new recombineering system for precise genome-editing in Shewanella oneidensis strain MR-1 using single-stranded oligonucleotides. Sci Rep. 9(1):39. doi: 10.1038/s41598-018-37025-4.

Wang, Q., A.A. Jones III, J.A. Gralnick, L. Lin, C.R. Buie. 2019. Microfluidic dielectrophoresis illuminates the relationship between microbial cell envelope polarizability and electrochemical activity. Sci Adv. 5(1):eaat5664. doi: 10.1126/sciadv.aat5664.


Conley, B.E., P.J. Intile, D.R. Bond and J.A. Gralnick. 2018. Divergent Nrf-family proteins and MtrCAB homologs facilitate extracellular electron transfer in Aeromonas hydrophila. Appl Environ Microbiol. doi: 10.1128/AEM.02134-18.

Philips J., N. Van den Driessche, K. De Paepe, A. Prévoteau, J.A. Gralnick, J.B.A. Arends and K. Rabaey. 2018. A novel Shewanella isolate enhances corrosion by using metallic iron as the electron donor with fumarate as the electron acceptor. Appl Environ Microbiol. 84(20):e00154-18 doi: 10.1128/AEM.01154-18.

Bennett B.D., K.E. Redford and J.A. Gralnick. 2018. Survival of anaerobic Fe2+ stress requires the ClpXP protease. J Bacteriol. 200(8):e00671-17 doi: 10.1128/JB.00671-17.

Bennett, B.D., K.E. Redford and J.A. Gralnick. 2018. The MgtE homolog FicI acts as a secondary ferrous iron importer in Shewanella oneidensis strain MR-1. Appl Environ Microbiol. 84(6):e01245-17 doi: 10.1128/AEM0.1245-17.

Rowe, A.R., P. Rajeev, A. Jain, S. Pirbadian, A. Okamoto, J.A. Gralnick, M.Y. El-Naggar and K.H. Nealson. 2018. Tracking electron uptake from a cathode into Shewanella cells: implications for energy acquisition from solid-substrate electron donors. mBio. 9(1):e02203-17. doi: 10.1128/mBio.02203-17.


West, E.A., A. Jain and J.A. Gralnick. 2017. Engineering a native inducible expression system in Shewanella oneidensis to control extracellular electron transfer. ACS Synth Biol. doi: 10.1021/acssynbio.6b00349

Bursac, T., J.A. Gralnick and J. Gescher. 2017. Acetoin production via unbalanced fermentation in Shewanella oneidensis. Biotechnol Bioeng. 114(6):1283-1289. doi: 10.1002/bit.26243


Badalamenti, J.P., Z.M. Summers, C.H. Chan, J.A. Gralnick and D.R. Bond. 2016. Isolation and genomic characterization of ‘Desulfuromonas soudanensis WTL’, a metal- and electrode-respiring bacterium from anoxic deep subsurface brine. Front Microbiol. 7:913. doi:10.3389/fmicb.2016.00913

Hajimorad, M. and J.A. Gralnick. 2016. Towards enabling engineered microbial electronic systems RK2-based conjugal transfer system for Shewanella synthetic biology. Electron Lett. 52(6):426-428.

Kane, A.L., E.D. Brutinel, H. Joo, R. Maysonet Sanchez, C.M. VanDrisse, N.J. Kotloski and J.A. Gralnick. 2016. Formate metabolism in Shewanella oneidensis generates proton motive force and prevents growth without an electron acceptor. J Bacteriol. 198(8):1337-1346.

Kane, A.L., B. Al-Shayeb, P.V. Holec, S. Rajan, N.E. Le Mieux, S.C. Heinsch, S. Psarska, K.G. Aukema, C.A. Sarkar, E.A. Nater and J.A. Gralnick. 2016. Toward bioremediation of methylmercury using silica encapsulated Escherichia coli harboring the mer operon. PLoS ONE 11(1):e0147036.


Bennett, B.D., E.D. Brutinel and J.A. Gralnick. 2015. A ferrous iron exporter mediates iron resistance in Shewanella oneidensis MR-1. Appl Environ Microbiol. 81(22):7938-44.

Bonis, B.M. and J.A. Gralnick. 2015. Marinobacter subterrani, a genetically tractable neutrophillic Fe(II)-oxidizing strain isolated from the Soudan Iron Mine. Front Microbiol. 6:719.


Yang, H., E.W. Krumholz, E.D. Brutinel, N.P. Palani, M.J. Sadowsky, A.M. Odlyzko, J.A. Gralnick and I.G. Libourel. 2014. Genome-scale metabolic network validation of Shewanella oneidensis using transposon insertion frequency analysis. PLoS Comput Biol. 10(9):e1003848.

Webster, D.P., M.A. TerAvest, D.F. Doud, A. Chakravorty, E.C. Holmes, C.M. Radens, S. Sureka, J.A. Gralnick and L.T. Angenent. 2014. An arsenic-specific biosensor with genetically engineered Shewanella oneidensis in a bioelectrochemical system. Biosens Bioelectron. 62:320-4.

TerAvest, M.A., M.A. Rosenbaum, N.J. Kotloski, J.A. Gralnick and L.T. Angenent. 2014. Oxygen allows Shewanella oneidensis MR-1 to overcome mediator washout in a continuously-fed bioelectrochemical system. Biotechnol Bioeng. 111(4):692-9.





Brutinel, E.D., A.M. Dean and J.A. Gralnick. 2013. Description of a riboflavin biosynthetic gene variant prevalent in the phylum Proteobacteria. J Bacteriol. 195(24):5479-86.

Kane, A.L., D.R. Bond and J.A. Gralnick. 2013. Electrochemical analysis of Shewanella oneidensis engineered to bind gold electrodes. ACS Synth Biol. 2(2):93-101.

Summers, Z.M., J.A. Gralnick and D.R. Bond. 2013. Cultivation of an obligate Fe(II)-oxidizing lithoautotrophic bacterium using electrodes. mBio. 4(1) e00420-12.

Kotloski, N.J. and J.A. Gralnick. 2013. Flavin electron shuttles dominate extracellular electron transfer by Shewanella oneidensis. mBio. 4(1) e00553-12. 


Gralnick, J.A. 2012. On conducting electron traffic across the periplasm. Biochem Soc Trans. 40(6):1178-80.

Brutinel, E.D. and J.A. Gralnick. 2012. Preferential utilization of D-lactate by Shewanella oneidensis. Appl Environ Microbiol. 78(23)8474-6.

Brutinel, E.D. and J.A. Gralnick. 2012. Anomalies of the anaerobic TCA cycle in Shewanella oneidensis revealed by Tn-seq. Mol Microbiol. 86(2):273-83.

Coursolle, D. and J.A. Gralnick. 2012. Reconstruction of extracellular respiratory pathways for iron(III) reduction in Shewanella oneidensis strain MR-1. Front Microbiol. 3:56.

Liu, N., Y. Xu, S. Hossain, N. Huang, D. Coursolle, J.A. Gralnick and E.M. Boon. 2012. Nitric oxide regulation of cyclic di-GMP synthesis and hydrolysis in Shewanella woodyi. Biochemistry. 51(10):2087-99.

Brutinel, E.D. and J.A. Gralnick. 2012. Shuttling Happens: soluble flavin mediators of extracellular electron transfer in Shewanella. Appl Microbiol Biotechnol. 93(1):41-8.

Flynn, C.M., K.A. Hunt, J.A. Gralnick and F. Srienc. 2012. Construction and elementary model analysis of a metabolic model for Shewanella oneidensis MR-1. Biosystems. 107(2):120-8.





Pinzon, N.M., K.G. Aukema, J.A. Gralnick and L.P. Wackett. 2011. Nile red detection of bacterial hydrocarbons and ketones in a high-throughput format. mBio. 2(4):e00109-11.

Sundararajan, A., J. Kurowski, T. Yan, D.M. Klingeman, M.P. Joachimiak, J. Zhou, B. Naranjo, J.A. Gralnick and M.W. Fields. 2011. Shewanella oneidensis MR-1 sensory box protein involved in aerobic and anoxic growth. Appl Environ Microbiol. 77(13):4647-56.

Jiao, Y., F. Qian, Y. Li, G. Wang, C.W. Saltikov and J.A. Gralnick. 2011. Deciphering the electron transport pathway for graphene oxide reduction by Shewanella oneidensis MR-1. J Bacteriol. 193(14):3662-5.

Cruz-Garcia, C., A.E. Murray, J.L. Rodrigues, J.A. Gralnick, L.A. McCue, M.F. Romine, F.E. Löffler and J.M. Tiedje. 2011. Fnr (EtrA) acts as a fine-tuning regulator of anaerobic metabolism in Shewanella oneidensis MR-1. BMC Microbiol. Mar;11:64.

Ross D.E., J.M. Flynn, D.B. Baron, J.A. Gralnick and D.R. Bond. 2011. Towards electrosynthesis in Shewanella: energetics of reversing the Mtr pathway for reductive metabolism. PLoS One. Feb; 6(2):e16649.


Flynn J.M., D.E. Ross, K.A. Hunt, D.R. Bond and J.A. Gralnick. 2010. Engineering unbalanced fermentations by using engineered electrode-interfaced bacteria. mBio. Nov;1(5):e00190-10.
mBiosphere Comment

Covington, E.D., C.B., Gelbmann, N.J. Kotloski and J.A. Gralnick. 2010. An essential role for UshA in processing of extracellular flavin electron shuttles by Shewanella oneidensis. Mol Microbiol. Oct; 78(2):519-32.

Coursolle, D. and J.A. Gralnick. 2010. Modularity of the Mtr respiratory pathway of Shewanella oneidensis strain MR-1. Mol Microbiol. Aug; 77(4):995-1008.

Johnson, E.T., D.B. Baron, B. Naranjo, D.R. Bond, C. Schmidt-Dannert and J.A. Gralnick. 2010. Light-driven proton pumping in an engineered bacterium enhances survival and electricity production. Appl Environ Microbiol. Jul; 76(13):4123-9.
Celebrating Ethan’s Life

Sukovich, D.J., J.L Seffernick, J.E. Richman, K.A. Hunt, J.A. Gralnick and L.P. Wackett. 2010. Head-to-head hydrocarbon biosynthetic genes in Shewanella oneidensis strain MR-1. Appl Environ Microbiol. Jun; 76(12):3842-9.

Sukovich, D.J., J.L. Seffernick, J.E. Richman, J.A. Gralnick and L.P. Wackett. 2010. Widespread head-to-head hydrocarbon biosynthesis in bacteria. Appl Environ Microbiol. Jun; 76(12):3850-62.

Hunt, K.A., J.M. Flynn, B. Naranjo, I.D. Shikhare and J.A. Gralnick. 2010. Substrate-level phosphorylation is the primary source of energy conservation during anaerobic respiraton of Shewanella oneidensis strain MR-1. J Bacteriol. Jul; 192(13):3345-51.

Coursolle, D., D.B. Baron, D.R. Bond and J.A. Gralnick. 2010. The Mtr respiratory pathway is essential for reducing flavins and electrodes in Shewanella oneidensis. J Bacteriol. Jan; 192(2):467-74.


Boal A.K., J.C. Genereux, P.A. Sontz, J.A. Gralnick, D.K. Newman and J.K. Barton. Redox signaling between DNA repair proteins for efficient lesion detection. 2009. Proc Natl Acad Sci U S A. Aug 31.

Baron, D.B., E. Labelle, D. Coursolle, J.A. Gralnick and D.R. Bond. 2009. Electrochemical measurement of electron transfer kinetics by Shewanella oneidensis MR-1. J Biol Chem. Oct 16; 284(42):28865-73.


Hau, H.H., A. Gilbert, D. Coursolle and J.A. Gralnick. 2008. Mechanism and Consequences of anaerobic respiration of cobalt by Shewanella oneidensis strain MR-1. Appl Environ Mircobiol. Nov;74(22):6880-6.

Marsili, E., D.B. Baron, I.D. Shikhare, D. Coursolle, J.A. Gralnick and D.R. Bond. 2008. Shewanella secretes flavins that mediate extracellular electron transfer. Proc Natl Acad Sci U S A. Mar 11; 105(10): 3968-3973.


Hau, H.H. and J.A. Gralnick. 2007. Ecology and biotechnology of the genus Shewanella. Ann Rev Microbiol. Jun 18; 61: 237 – 258.

Gralnick, J.A. and D.K. Newman. 2007. Extracellular Respiration. Mol Microbiol. July; 65(1): 1-11.


Gralnick, J.A., H. Vali, D.L. Lies and D.K. Newman. 2006. Extracellular respiration of dimethyl sulfoxide by Shewanella oneidensis strain MR-1. Proc Natl Acad Sci U S A. Mar 21; 103(12): 4669-4674.


Newman, D.K. and J.A. Gralnick. 2005. What genetics offers geobiology. Reviews in Mineralogy and Geochemistry. V. 59; p. 9-26.

Kappler A., Emerson D., Edwards K., Amend J.P., Gralnick J.A., Grathwohl P., Hoehler T., Straub K.L., 2005. Microbial activity in biogeochemical gradients - new aspects of research. Geobiology, 3, 229-233.