24th Annual Bollum Symposium

"Celebrating the Legacy of Fred Bollum, Excellence in Graduate Research"

Wednesday, May 3, 2023
1:00-5:00 PM
2-101 Nils Hasselmo Hall
Minneapolis Campus


1:00 p.m. Welcome & Introductions, Dr. David Bernlohr
1:10 p.m. Ancient genetic conflicts with the host innate immune system have shaped herpesvirus evolution in primates, Sofia Moraes
2:00 p.m. Clearing hurdles: understanding natural virus transmission and evolution at barrier surfaces, Shanley Roach
2:50 p.m.  Intermission
3:20 p.m. Autoregulation of G protein-coupled receptor signaling through the third intracellular loop, Fredrik Sadler
4:10 p.m. Biochemical and structural interrogation of a borosin RiPP system, Kathryn Crone


Background for the Frederick J. Bollum Endowed Research Fund for Biochemistry Lectureship Series

Frederick J. Bollum received a B.A. in Zoology in 1949 and a Ph.D. in Physiological Chemistry in 1956 from the University of Minnesota. He went on to a Postdoctoral Fellowship at the Universtiy of Wisconsin and then took a position at Oak Ridge National Laboratory in Tennessee. Later, he became a Professor of Biochemistry at the University of Kentucky Medical School, in Lexington, KY and then Chairman of Biochemistry at Uniformed Services University for the Health Sciences, Bethesda, MD. He then moved on to become President and CEO of Supertechs, Inc., Biotechnology Consultants. His major research interests were nucleic acid chemistry and enzymology, nucleotide metabolism, genetic aspects of biochemistry, immunological diversification and recombinant DNA, authoring more than 260 papers and three books.

Dr. Bollum regularly contributes to the Department of Biochemistry, Molecular Biology and Biophysics through the Minnesota Medical Foundation. His gifts have helped support lectures, research, graduate student awards and meeting opportunities and an annual symposium on cutting edge research topics.




Unnatural Biology
Re-imagining the form and function of living cells

Dr. Jason Chin

Dr. Farren Isaacs

Dr. Floyd Romesberg

Dr. Tanja Kortemme

Reprogramming the genetic code

Genomes by design: Recoding & rewriting life’s genetic blueprint

Semi-synthetic organisms that store and retrieve increased genetic information

Sensors and new shapes: Computational design of new molecular geometries and ligand-controlled functions


Coupling Metabolism & Protein Post-translational Modifications


Dr. Yingming Zhao, University of Chicago 

Dr. Sumin Kang, Emory University

Dr. Hening Lin, Cornell University

Dr. Kathryn Wellen, University of Pennsylvania

"A new paradigm of epigenetic regulation linking cell. metab. to histone marks"

"Phosphorylation in metabolic signaling and cancer progression"

"Regulatory roles of metabolites"

"Acetyl-CoA metabolism in chromatin regulation and tumorigenesis"




Brenton Hoffman, PhD, Duke University

Marcos Sotomayor, PhD, Ohio State University

Matt Lang, PhD, Vanderbilt University

Arpita Upadhyaya, PhD, University of Maryland

Scott Manalis, PhD, Massachusetts Institute of Technology

"Assessing the effects of protein load on protein function"

"Molecular mechanics of hearing"

"Single molecule drivers of αβ T cell recognition"

"Cytoskeletal dynamics of mechanosensing in immune cells"

"Non-invasive monitoring of single-cell mechanics"


Versatile Functions of RNA in Biology and Disease



Hashim AL Hashikmi, Ph.D.

Duke University

Lynne Maquat, Ph.D.

University of Rochester

Kristen Lynch, Ph.D.

University of Pennsylvania

Maurice Swanson, Ph.D.

University of Flordia

"Dynamic RNA Structures in Translational Fidelity and Drug Discovery"

"Nonsense-mediated mRNA Decay and Human Disease: Genome Guardian and Executor"

"Getting Sick of Splicing: Alternative Splicing and the Human Immune System"

"RNA-mediated Mechanisms in Development and Disease"


Therapeutic Proteins: a Protein Engineering Perspective


Jonathan Dordick, Ph.D.

Center for Biotechnology & Interdisciplinary Studies

Joanna Swain, Ph.D.

Bristol-Myers Squibb

Eric Gaucher, Ph.D.

Parker H. Petit Institute for Bioengineering & Bioscience

Hans Erickson, Ph. D.


"Biomolecular engineering as an emerging paradigm for advancing medicine"

"In vitro selection coupled with NGS in therapeutic protein discovery at Bristol-Myers Squibb"

"Engineering ancient proteins for biotherapeutic development"

"Building better antibody-drug conjugates for the treatment of cancer"


Biochemistry Centennial Celebration


Dr. Hung-Ying Kao (Ph.D., 1995)

Dr. Amy Rocklin (Ph.D., 2000)

Dr. Venkateswarlu Pothapragada (Ph.D., 1962)

Dr. Brad Wallar (Ph.D., 2000)

Dr. Rebecca Moen (Ph.D., 2013)

Dr. Melanie Simpson (Ph.D., 1997)

"Identification of Prp40, a Novel Essential Yeast Splicing Factor Associated With the U1 snRNP"

"Structural and Mechanistic Studies of 1-Aminocyclopropane-1-Carboxylic Acid Oxidase"

"Studies on Fluoride Metabolism and Transport"

"Probing The Interactions Between Component B and The Hydroxylase Of Methane Monooxygenase"

"Site-directed Modifications of Myosin"

"Biochemical and Physiological Analysis of the Adipocyte Lipid-binding Protein"


The dynamic microbiome

Jose Carlos Clemente, PhD

Mount Sinai School of Medicine

Lawrence David, PhD

Duke University

Dan Knights, PhD

University of Minnesota-Twin Cities

Michael Sadowsky,

University of Minnesota-Twin Cities

"From population studies to inflammation and cancer: the microbiome in health and disease"

"Perturbing Human Microbiota"

"Linking microbiome structure and function to host phenotype and genotype"

"Use of Fecal Microbiota Therapeutics to Treat and Understand Intestinal Dysbiosis"


Discovery Metabolomics

Oliver Fiehn, PhD

University of California, Davis

Ayyalusamy Ramamoorthy, PhD

University of Michigan

Jared Rutter, PhD

University of Utah School of Medicine

Gary Siuzdak, PhD

The Scripps Research Institute

"The NIH West Coast Metabolomics Center: Integrating metabolomics with genomic data"

"NMR-based Metabolomics for Cancer Biomarker Discovery"

"The Integrated Regulation of Metabolism"

"Mass Spectrometry-Based Global Metabolomics for Understanding Disease Pathogenesis"


Structure, Motion, Biomarkers: Discovery through NMR


Prof. Dorothee Kern, Brandeis University

Prof. Jeremy K. Nicholson, Imperial College, London

Prof. Chad M. Rienstra, University of Illinois

Prof. Gerhard Wagner, Harvard University

Choreographing an enzyme's dance-dynamics during catalysis

Spectroscopy and Stratified Medicine: Getting Systems Biology into the Clinic

Taking solid-state NMR to extremes: membrane proteins, fibrils, new methods

New NMR experiments for challenging proteins


Biophysics of Nucleic Acid-Protein Interactions


Dr. Michael Fried, University of Kentucky, Lexington, KY

Dr. Ioulia Rouzina, University of Minnesota, Minneapolis, MN

Dr. Phillip A. Sharp, Massachusetts Institute of Technology, Cambridge, MA

Dr. Mark C. Williams, Northeastern University, Boston, MA

O6 Alkylguanine-DNA Alkyltransferase. New Functions For A Guardian of the Genome.

Bloomfield-Inspired Biophysics in HIV Research

From Biophysics to RNA Biology

Mechanochemistry of DNA binding: From small molecules to proteins


Unconventional Nucleic Acid Biology

Dr. Brenda Bass, University of Utah, Salt Lake City, UT

Dr. Ronald Breaker, Yale University, New Haven, CT

Dr. Simon Wain-Hobson, Pasteur Institute, Paris, France

Dr. Michael Neuberger, University of Cambridge, Cambridge, UK

Toward a mechanistic understanding of Dicer's different roles in processing dsRNA.

Riboswitches- Molecular Relics from the RNA World?


The pros and cons of viral hypermutation.


Antibody diversification through DNA deamination.


The Biochemistry of Biofuels


Dr. Timothy Donohue, University of Wisconsin-Madison

Dr. Jay Keasling, University of California, Berkeley

Dr. James C. Liao, University of California, Los Angeles

Dr. Stephen Ragsdale, University of Michigan, Ann Arbor

Tapping into Microbial Genomes for Light-driven Hydrogen Production.

Engineering Microorganisms for Production of Advanced Biofuels.

Fuels and Chemical Beyond Petroleum.

Nickel Enzymes in the Biochemistry of Biofuels.



Circadian Clocks

Dr. Steve A. Kay, University of California, San Diego

Dr. Amita Sehgal, HHMI/ University of Pennsylvania Medical School

Dr. Joseph S. Takahashi, HHMI/Northwestern University

Dr. Ying-Hui Fu, University of California, San Francisco

Network Discovery Pipelines for Circadian Clocks.

Molecular Biology of Circadian Rhythms in Drosophila.

Genetic Analysis of Circadian Clocks in Mammals.

Molecular Characterization of Human Sleep Variants.


Reguating Biological Function by Ubiquitination

Dr. Rachel Klevit, University of Washington, Seattle, WA

Dr. Keith D. Wilkinson, Emory University School of Medicine, Atlanta, GA

Dr. Daniel Finley, Harvard Medical School, Boston, MA

Dr. Randy Hampton, University of California, San Diego, La Jolla, CA

Recognition and Disassembly of the Polyubiquitin Targeting Signal.

Structural Insights into Diversity and specificity in Protein Ubiquitination.

Regulation of Protasome Activity.

Protein Quality Control in Cell Regulation.


The Science of Aging

Dr. Leonard P. Guarante, MIT, Boston

Dr. Holly Van Remmen, University of Texas, San Antonio

Dr. Richard A. Miller, University of Michigan

Dr. Richard Weindruch, University of Wisconsin

SIR2, Calorie Restriction and Aging

Fifty Years of the Oxidative Stress Theory of Aging: Where Are We Now? Fifty Years of the Oxidative Stress Theory of Aging: Where Are We Now?

Size, Stress, and Aging: Lessons from Dwarf Mice

Caloric Restriction and Aging: Studies in Mice and Monkeys


Computational Biochemistry and Molecular Biology

Dr. Eric D. Siggia, Rockefeller University in New York

Dr. Peter D. Karp, Stanford Research Institute International (SRI), Palo Alto (CA)

Dr. Michael A. Savageau, University California, Davis.

Dr. Jiali Gao, University of Minnesota

Computation Approaches to Blastoderm Patterning in the Fly and its Evolution.

Databases and Algorithms for Pathway Bioinformatics.

System Design Principles and Construction of Gene Circuits

Dynamics, Pathways, and Tunneling: A Computational Perspective of Enzyme Catalysis


Proteomics: Advancing Our Understanding of Biology


Michel Desjardins, Université de Montréal

Tim Haystead,Duke University Medical Center

Kathryn E. Howell
University of Colorado School of Medicine, Denver

Bradford W. Gibson
Buck Institute of Aging Research

Proteomics Brings New Paradigms to Immunology

Proteome Mining Drug and Target Discovery en Masse.

New Insights into Golgi Function Through Proteomics

The Mitochondrial Proteome, Neurodegenerative Disease and Aging


Structural Dymamics of Membrane Signal Transduction


Dr. Wayne Hubbell, Jules Stein Eye Institute, UCLA

Dr. Lynmarie Thompson, University of Massachusetts, Amherst

Dr. John Spudich, University of Texas, Houston

Dr. David Farrens, Oregon Health and Science University

A nitroxide's view of rhodopsin structure, dynamics and activation

Site-directed solid-state NMR probing transmembrane signaling mechanisms in bacterial chemoreceptors.

Microbial rhodopsins: structure/function in ion transport and signaling.

Conformational changes in G-protein coupled receptors: insights gained from site-directed fluorescence studies.


Nature's Copier: the DNA Replication Machinery

Dr. Melvin DePamphilis, National Institute of Child Health and Human Development

Dr. Bik-Kwoon Tye, Cornell University

Dr. James Berger, University of California, Berkeley

The ORC Cycle: a Novel Mechanism for Regulating Eukaryotic DNA Replication

Coordination of Genome Expression and DNA Replication in Proliferating Cells: The Multiple Roles of the MCM Proteins in Eukaryotes

Toward a Structural Understanding of DNA Replication Initiation


Directed Evolution of Biomolecules & Functional Genomics

Dr. Jeremy Minshull, Maxygen

Dr. Karl Dane Wittrup, Massachusetts Institute of Technology

Dr. George Georgiou, University of Texas


Biological Diversity: From Nature and Beyond.

Engineering Protein Recognition, Stability, and Expression by Yeast Display

Functional Expression of Multidisulfide Proteins in
Bacteria: Genetic, Biochemical, Structural Biology and Directed Evolution Strategies.


G Protein-Coupled Signaling

Nigel Bunnett, University of California, San Francisco

Linda Hicke, Northwestern University

John Tesmer, University of Texas

David Clapham, Harvard Medical School

How Proteases Talk to Cells:   A Comparison of Signaling by Proteases and Neuropeptides

Role of Ubiquitin in Signaling Receptor Downregulation

Effector Regulation by Heterotrimeric G   Proteins:   Lessons Learned from Adenyl Cyclase

Protein Gated Potassium Channels


Transcription Factors & Metabolic Control

Gretchen J. Darlington, Baylor College of Medicine

Steven A. Kliewer, Glaxo-Wellcome Research & Development

Timothy S. Osborne, University of California, Irvine

The Role of C/EBP Proteins in Integratie Metabolism

PPARs and PXR: Orphan Nuclear Receptors that Define Novel Hormone Signaling Pathways.

Positive and Negative Regulation of Genes that Regulate Cholesterol Metabolism.