Thomas Lab

Research Facilities

Biophysical Technology Center

• Electron Paramagnetic Resonance (EPR)
  • Steady-state (continuous wave X-band)
  • Time-resolved (pulsed X-band, DEER)
• Fluorescence and Luminescence Resonance Energy Transfer (FRET and LRET)
  • Steady-state (Eclipse, Gemini)
  • Time-resolved (Direct Waveform Recording, Single Photon Counting, DWR Fluorescence Lifetime Plate Reader)
• Time-resolved Phosphorescence Anisotropy (TPA)
•  Drug discovery
  • Liquid dispensers
  • Compound libraries (1, 3 and 50k) in 384 and 1536well plates
  • Consulting on FRET-based biosensor design

Nuclear Magnetic Resonance Facility

• Collaborator: Gianluigi Veglia

Techniques

Computation

• High-throughput data acquisition and analysis
  • Andrew Thompson
• Molecular dynamics simulations
  • Bengt Svensson
  • Collaborators: Jonathan Sachs

Crystallography

• Spectroscopy and x-ray diffraction on labeled protein crystals
  • Nanoliter Crystallization Facility 
  • Kahlert Structural Biology Laboratory 
  • Collaborator: Hideki Aihara, BMBB Professor and Director of NCF and KSBL

Enzyme Kinetics

• Correlate biochemical and molecular dynamics
• Transient kinetics using laser flash photolysis or stopped flow
• Transient Time-resolved FRET

Physiology

• ATPase assays
• Muscle mechanics 
• Effects of aging, muscular dystrophy
  • Collaborator: Dawn Lowe

Protein Engineering

• Molecular Biology:
  • Site-directed mutagenesis
  • site-directed labeling
  • fluorescent fusion proteins
• Cell culture: bacteria, Dictyostelium, insect, yeast, mammalian