The Fluorescence Lifetime Plate Reader (FLTPR), designed in collaboration with Fluorescence Innovations, Inc., uses technology similar to that of the TRF instrument in Fargoland, to perform high-throughput time-resolved fluorescence lifetime measurements in a microplate format (96, 384 or 1536 wells).
The FLTPR is currently equipped with microchip laser excitation at 473 and 532 nm. With the ability to read a 1536 well plate in 2 minutes, this instrument is truly HTS compatible. We have demonstrated use with 50,000 compound library screens.
Selected publications of instrument use:
- Bunch TA, Guhathakurta P, Thompson AR, Lepak VC, Carter AL, Thomas JJ, Thomas DD, Colson BA. Drug discovery for heart failure targeting myosin-binding protein C. J Biol Chem. 2023 Dec;299(12):105369. doi: 10.1016/j.jbc.2023.105369. Epub 2023 Oct 20. PMID: 37865311; PMCID: PMC10692721.
- Braun AR, Kochen NN, Yuen SL, Liao EE, Cornea RL, Thomas DD, Sachs JN. Advancements in a FRET Biosensor for Live-Cell Fluorescence-Lifetime High-Throughput Screening of Alpha-Synuclein. ASN Neuro. 2023 Jan-Dec;15:17590914231184086. doi: 10.1177/17590914231184086. PMID: 37428128; PMCID: PMC10338669.
- Nikolaienko R, Bovo E, Solberg JC, Brinkmann M, Treinen LM, Thompson AR, Berg K, Thomas DD, Thomas JJ, Bers DM, Aldrich CC, Zima AV, Cornea RL, Rebbeck RT. Large-scale high-throughput screen for cardiac ryanodine receptor targeted therapeutics. J Biol Chem. 2026 Jan;302(1):110951. doi: 10.1016/j.jbc.2025.110951. Epub 2025 Nov 17. PMID: 41260336; PMCID: PMC12765055.
Acknowledgements
The BTC should be acknowledged in any publications arising from the data analyzed in the BTC with the following text:
Experiments reported in this paper were performed at the Biophysical Technology Center, University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics.
Once published, please let us know via an email to [email protected].