The Fluorescence Lifetime Plate Reader (FLPR), designed in collaboration with Fluorescence Innovations, Inc., uses technology similar to that of the TRF instrument in Fargoland, to perform high-throughput time-resolved fluorescence lifetime measurements in a microplate format (96 or 384 wells).
The FLPR is currently equipped with microchip laser excitation at 532 nm and 355 nm, and it records 384 nanosecond time-resolved waveforms within 10 min, the same speed as a conventional intensity-based plate reader, with 30 times better precision.
Selected publications of instrument use:
- Muretta, JM, A Kyrychenko, AS Ladokhin, DJ Kast, GD Gillispie, and DD Thomas. 2010. High -performance time-resolved fluorescence by direct waveform recording. Rev Sci Instrum 81:103101 (1-8).
- Rebbeck, RT, MM Essawy, FR Nitu, BD Grant, GD Gillispie, DD Thomas, DM Bers, and RL Cornea. 2017. High-throughput screens to discover small-molecule modulators of ryanodine receptor calcium release channels. SLAS Discov 22(2):176-186.
Acknowledgements
The BTC should be acknowledged in any publications arising from the data analyzed in the BTC with the following text:
Experiments reported in this paper were performed at the Biophysical Technology Center, University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics.
Once published, please let us know via an email to umn-btc@umn.edu.