Gemini EM is a microplate spectrofluorometer from Molecular Devices. It has dual scanning monochromators that allow for 1 nm increment selection over the wavelength range of 250 - 850 nm. Bandwidth for both excitation and emission is 9 nm. Lamp source is a Xenon flash lamp (1 Joule/flash). Scanning options are endpoint, kinetic, spectral scanning, and well scanning. Microplate formats include 6-384 well plates. Top and bottom readings are available. Additional features include shaker and temperature control.
Light Source: Xenon flash lamp
Wavelength range: Excitation: 250-850 nm, Emission: 360-850 nm
Wavelength Selection: Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth (Excitation and Emission): 9 nm
Dynamic range: 10^6
Range: Ambient + 4 °C up to 45 °C
Uniformity (Microplate): ± 1 °C @ 37 °C
Computer software is Molecular Devices SoftMax® Pro. Features include five parameter logistic curve fitting, parallel line analysis with observation weighting, and hundreds of ready to run assay protocols.
Selected publications of instrument use:
- Svensson, B, T Oda, FR Nitu, Y Yang, I Cornea, Y Chen-Izu, JD Fessenden, DM Bers, DD Thomas, and RL Cornea. 2014. FRET-based trilateration of probes bound within functional ryanodine receptors. Biophys J 107:2037-2048. Cover article.
- Cornea, RL, SJ Gruber, EL Lockamy, JM Muretta, D Jin, J Chen, R Dahl, T Bartfai, KM Zsebo, GD Gillispie, and DD Thomas. 2012. High-throughput FRET assay yields allosteric SERCA activators. J Biomol Screen 18:97-107.
- Cornea, RL, FR Nitu, M Samso, DD Thomas and BR Fruen. 2010. Mapping the ryanodine receptor FK506-binding protein subunit using fluorescence resonance energy transfer. J Biol Chem 285:19219-19226.
The BTC should be acknowledged in any publications arising from the data analyzed in the BTC with the following text:
Experiments reported in this paper were performed at the Biophysical Technology Center, University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics.
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