The Spectramax 384Plus is a microplate spectrofluorometer from Molecular Devices. Regularly used for NADPH ATPase assays, and BCA assays
Light Source: Xenon flash lamp
Wavelength range: Excitation: 250-850 nm, Emission: 360-850 nm
Wavelength Selection: Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth (Excitation and Emission): 9 nm
Dynamic range: 10^6
Temperature:
Range: Ambient + 4 °C up to 45 °C
Uniformity (Microplate): ± 1 °C @ 37 °C
Computer software is Molecular Devices SoftMax® Pro7, which Features include five parameter logistic curve fitting, parallel line analysis with observation weighting, and hundreds of ready to run assay protocols.
Selected publications of instrument use:
- Schaaf, TM, E Kleinboehl, SL Yuen, LN Roelike, B Svensson, AR Thompson, RL Cornea, and DD Thomas. 2020. Live-Cell Cardiac-Specific High-Throughput Screening Platform for Drug-Like Molecules that Enhance Ca2+ Transport. Cells 9:1170.
- Stroik, DR, SL Yuen, KA Janicek, TM Schaaf, J Li, DK Ceholski, RJ Hajjar, RL Cornea, and DD Thomas. 2018. Targeting protein-protein interactions for therapeutic discovery via FRET-based high-throughput screening in living cells. Nature Sci Rep 8:12560.
- Schaaf, TM, KC Peterson, BD Grant, P Bawaskar, S Yuen, J Li, JM Muretta, GD Gillispie, and DD Thomas. 2017. High-throughput spectral and lifetime-based FRET screening in living cells to identify small molecule effectors of SERCA. SLAS Discov 22:262-273.
Acknowledgements
The BTC should be acknowledged in any publications arising from the data analyzed in the BTC with the following text:
Experiments reported in this paper were performed at the Biophysical Technology Center, University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics.
Once published, please let us know via an email to [email protected].