The Spectramax 384Plus is a microplate spectrofluorometer from Molecular Devices. Regularly used for NADPH ATPase assays, and BCA assays
Light Source: Xenon flash lamp
Wavelength range: Excitation: 250-850 nm, Emission: 360-850 nm
Wavelength Selection: Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth (Excitation and Emission): 9 nm
Dynamic range: 10^6
Range: Ambient + 4 °C up to 45 °C
Uniformity (Microplate): ± 1 °C @ 37 °C
Computer software is Molecular Devices SoftMax® Pro7, which Features include five parameter logistic curve fitting, parallel line analysis with observation weighting, and hundreds of ready to run assay protocols.
Selected publications of instrument use:
- Schaaf, TM, E Kleinboehl, SL Yuen, LN Roelike, B Svensson, AR Thompson, RL Cornea, and DD Thomas. 2020. Live-Cell Cardiac-Specific High-Throughput Screening Platform for Drug-Like Molecules that Enhance Ca2+ Transport. Cells 9:1170.
- Stroik, DR, SL Yuen, KA Janicek, TM Schaaf, J Li, DK Ceholski, RJ Hajjar, RL Cornea, and DD Thomas. 2018. Targeting protein-protein interactions for therapeutic discovery via FRET-based high-throughput screening in living cells. Nature Sci Rep 8:12560.
- Schaaf, TM, KC Peterson, BD Grant, P Bawaskar, S Yuen, J Li, JM Muretta, GD Gillispie, and DD Thomas. 2017. High-throughput spectral and lifetime-based FRET screening in living cells to identify small molecule effectors of SERCA. SLAS Discov 22:262-273.
The BTC should be acknowledged in any publications arising from the data analyzed in the BTC with the following text:
Experiments reported in this paper were performed at the Biophysical Technology Center, University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics.
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