The spectral unmixing plate reader, designed in collaboration with Fluorescence Innovations, Inc., acquired fluorescence spectra in a microplate format (96, 384 or 1536 wells).
The SUPR is currently equipped with laser excitation at 473 and 532nm. With the ability to read a 1536 well plate in 8 minutes, this instrument is truly HTS compatible. We have demonstrated use in screening to identify compound fluorescence, and to monitor structural transitions of target proteins.
Selected publications of instrument use:
- Bunch TA, Guhathakurta P, Thompson AR, Lepak VC, Carter AL, Thomas JJ, Thomas DD, Colson BA. Drug discovery for heart failure targeting myosin-binding protein C. J Biol Chem. 2023 Dec;299(12):105369. doi: 10.1016/j.jbc.2023.105369. Epub 2023 Oct 20. PMID: 37865311; PMCID: PMC10692721.
- Baker ZD, Thompson AR, Thomas DD, Levinson NM. Tyrosine-Mediated Static and Dynamic Quenching of a Receptor Tyrosine Kinase Biosensor Reveals Inhibitor Binding Modes and Kinase Conformations. ACS Chem Biol. 2025 Jul 18;20(7):1683-1695. doi: 10.1021/acschembio.5c00224. Epub 2025 Jun 19. PMID: 40536894; PMCID: PMC12281483.
- Nikolaienko R, Bovo E, Solberg JC, Brinkmann M, Treinen LM, Thompson AR, Berg K, Thomas DD, Thomas JJ, Bers DM, Aldrich CC, Zima AV, Cornea RL, Rebbeck RT. Large-scale high-throughput screen for cardiac ryanodine receptor targeted therapeutics. J Biol Chem. 2026 Jan;302(1):110951. doi: 10.1016/j.jbc.2025.110951. Epub 2025 Nov 17. PMID: 41260336; PMCID: PMC12765055.
Acknowledgements
The BTC should be acknowledged in any publications arising from the data analyzed in the BTC with the following text:
Experiments reported in this paper were performed at the Biophysical Technology Center, University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics.
Once published, please let us know via an email to [email protected].